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Series GSE174592 Query DataSets for GSE174592
Status Public on May 03, 2024
Title MCF7 cells with LIFR knockdown
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary MCF7 cells were infected with lentiviral particles containing LIFR-targeted shRNAs then chemically selected to create a stable pooled population of shLIFR MCF7 cells. The goal of the study was to determine the downstream targets of LIFR in human MCF7 breast cancer cells.
 
Overall design There are three biological replicates of the control MCF7 cells expressing non-silencing control shRNAs, and three biological replicates of the MCF7 cells expressing LIFR shRNAs. Each biological replicate was collected from independent experiments and all cells were grown in standard 2D tissue culture plates.
 
Contributor(s) Johnson RW
Citation(s) 34247191
Submission date May 17, 2021
Last update date May 03, 2024
Contact name Rachelle Johnson
Organization name Vanderbilt University Medical Center
Street address 2215B Garland Ave
City Nashville
ZIP/Postal code 37232
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM5320213 MCF7NSC 2D BR1
GSM5320214 MCF7NSC 2D BR2
GSM5320215 MCF7NSC 2D BR3
Relations
BioProject PRJNA730596
SRA SRP320230

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174592_MultiRankSeq_222324_vs_192021.html.diff.xlsx 4.6 Mb (ftp)(http) XLSX
GSE174592_Raw_RNAseq_counts_MCF7shLIFR_2D_samples.xlsx 2.1 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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