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| Status |
Public on Mar 12, 2024 |
| Title |
Genome-wide RNAseq of liver tissue derived from humanized mice fed by CDA-HFD and treated with CLDN1 mAb or vehicle Control |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Six-week old Fah−/−/Rag2−/−/Il2rg −/− (FRG) –NOD breeding mice were intravenously injected with 1.5 x 10^9 plaque forming units (pfu) of an adenoviral vector encoding the secreted form of the human urokinase-like plasminogen activator (Ad-uPA). Forty-eight hours later, 10^6 PHH were injected intrasplenically. Successfully transplanted mice were fed with CDA-HFD for 16 weeks and then treated with humanized CLDN1 specific mAb 500 µg or vehicle for additional 8 weeks. RNA was extracted from non-tumorous liver tissue of 4 NASH fibrosis mice treated with CLDN1 mAb, 3 vehicle Control treated NASH fibrosis mice as well as 3 humanized mice fed by regular diet. Libraries were sequenced on the Illumina HiSeq 4000 as Single-Read 50 base reads.
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| Overall design |
mRNA profiles of humanized mice fed a regular diet, humanized NASH fibrosis mice treated with vehicle control and humanized NASH fibrosis mice treated with CLDN1 mAb
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| Contributor(s) |
El Saghire H, Jühling F, Saviano A, Röhlen N, Baumert TF |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 17, 2021 |
| Last update date |
Mar 12, 2024 |
| Contact name |
Frank Jühling |
| E-mail(s) |
juehling@unistra.fr
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| Organization name |
Université de Strasbourg, Inserm, Institut de Recherche sur Les Maladies Virales et Hépatiques UMR_S1110
|
| Street address |
3 Rue Koeberlé
|
| City |
Strasbourg |
| ZIP/Postal code |
67000 |
| Country |
France |
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| Platforms (1) |
| GPL25431 |
Illumina HiSeq 4000 (Homo sapiens; Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA730507 |
| SRA |
SRP320107 |
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