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| Status |
Public on Apr 07, 2022 |
| Title |
The splicing factor Prpf31 is required for hematopoietic stem and progenitor cell expansion during zebrafish embryogenesis |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Pre-mRNA splicing is a precise regulated process, and is crucial for system development and homeostasis maintenance. Mutations in spliceosomal components have been found in various hematopoietic malignancies (HMs), and have been considered as oncogenic derivers of HMs. However, the role of spliceosomal components in normal and malignant hematopoiesis remain largely unknown. Pre-mRNA processing factor 31 (PRPF31) is a constitutive spliceosomal component, which mutations are associated with autosomal dominant retinitis pigmentosa. PRPF31 was found to be mutated in several HMs, but the function of PRPF31 in normal hematopoiesis has not been explored. In this study, we generated a prpf31 knockout zebrafish line, and discovered that prpf31 mutants exhibited severe defects in hematopoietic stem and progenitor cell (HSPC) expansion and its sequentially differentiated lineages. Immunofluorescence results showed that Prpf31 deficient HSPCs underwent malformed mitosis and M phase arrest during HSPC expansion. Transcriptome analysis and experimental validations revealed that Prpf31 deficiency extensively perturbed the alternative splicing of mitosis-related genes. Collectively, our findings elucidate a previously undescribed role for Prpf31 in HSPC expansion, through regulating the alternative splicing of mitosis-related genes.
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| Overall design |
RNA-seq profiles of wild-type sibling and prpf31-/- zebrafish embryos at 36 hours post fertilization for triplicates
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| |
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| Contributor(s) |
Lv Y, Li J, Liu M |
| Citation(s) |
38382674 |
| Submission date |
Apr 07, 2021 |
| Last update date |
Apr 05, 2024 |
| Contact name |
Yuexia Lv |
| Organization name |
Huazhong University of Science and Technology
|
| Department |
College of Life Science and Technology
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| Lab |
Key Laboratory of Molecular Biophysics of the Ministry of Education
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| Street address |
Hubei, Wuhan, Hongshan district, Luoyu Road, Huazhong University of Science and Technology
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430074 |
| Country |
China |
| |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA720429 |
| SRA |
SRP313872 |
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