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| Status |
Public on Jun 25, 2009 |
| Title |
C. elegans fer-1 mutant gene expression profile |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
In vertebrate muscle, loss of Dysferlin results in the activation of compensatory muscle gene expression, even at pre-pathological stages. We hypothesized that if C. elegans fer-1 is also expressed in muscle, then fer-1 mutant worms might also exhibit compensatory muscle gene expression.
To test this hypothesis, we used Affymetrix microarrays to profile gene expression from synchronized wild type and fer-1 mutant adults. To improve the specificity of this approach, we profiled two well characterized loss-of-function fer-1 mutants (hc1ts and hc24ts) and considered genes that changed similarly in both mutants as fer-1-regulated transcripts.
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| Overall design |
For each genotype, six individual replicates were performed and were hybridized to Affymetrix C. elegans Genechips using the manufacturer’s recommended protocols. The best four preparations (as determined by the overall Pearson Correlation within a genotype) were used for RNA labeling and hybridization.
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| Contributor(s) |
Khurana TS, Lamitina T, Krajacic P |
| Citation(s) |
19755517 |
| Submission date |
Jun 23, 2009 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Predrag Krajacic |
| Organization name |
University of Pennsylvania
|
| Street address |
3700 Hamilton Walk A700 Richards
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (12)
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| GSM419959 |
Wild-type young adult biological rep 3 |
| GSM419960 |
Wild-type young adult biological rep 4 |
| GSM419961 |
Wild-type young adult biological rep 5 |
| GSM419962 |
Wild-type young adult biological rep 6 |
| GSM419963 |
fer-1 (hc1ts) mutant young adult biological rep 1 |
| GSM419964 |
fer-1 (hc1ts) mutant young adult biological rep 2 |
| GSM419965 |
fer-1 (hc1ts) mutant young adult biological rep 5 |
| GSM419966 |
fer-1 (hc1ts) mutant young adult biological rep 6 |
| GSM419967 |
fer-1 (hc24ts) mutant young adult biological rep 1 |
| GSM419968 |
fer-1 (hc24ts) mutant young adult biological rep 2 |
| GSM419969 |
fer-1 (hc24ts) mutant young adult biological rep 3 |
| GSM419970 |
fer-1 (hc24ts) mutant young adult biological rep 5 |
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| Relations |
| BioProject |
PRJNA117459 |
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