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| Status |
Public on Dec 24, 2023 |
| Title |
DNA methylation of fibroblasts from Cockayne syndrome and UV-sensitivity syndrome patients [450k] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Cockayne syndrome (CS) is a rare genetic disorder caused by mutation of the DNA repair and chromatin remodelling proteins CSA or CSB. Increasing evidences indicate that the progeroid phenotype of CS cannot be solely ascribed to impaired DNA repair, and UV-sensitivity syndrome (UVSS) patients that are also mutated for CSA or CSB do not age prematurely. Epigenetic modifications constitute a hallmark of ageing. We assessed genome-wide DNA methylation (DNAm) at single-nucleotide resolution on fibroblasts derived from CS versus UVSS patients and healthy donors.
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| Overall design |
1 µg of DNA was bisulfite-converted using the EZ DNA Methylation kit (Zymo Research) and analysed using the Infinium HumanMethylation450 or the HumanMethylationEPIC BeadChip (Illumina) following manufacturer's instructions
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| Contributor(s) |
Crochemore C, Sarasin A, Ricchetti M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 24, 2020 |
| Last update date |
Dec 25, 2023 |
| Contact name |
Maria Giulia Bacalini |
| E-mail(s) |
mugiu17@hotmail.com
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| Phone |
00390512094748
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| Organization name |
IRCCS Istituto delle Scienze Neurologiche di Bologna
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| Department |
Brain Aging Laboratory
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| Street address |
Via Altura 3
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| City |
Bologna |
| ZIP/Postal code |
40139 |
| Country |
Italy |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (11)
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| GSM4988100 |
genomic DNA from fibroblasts from donor 911VI |
| GSM4988101 |
genomic DNA from fibroblasts from donor CS177VI |
| GSM4988102 |
genomic DNA from fibroblasts from donor 194VI |
| GSM4988103 |
genomic DNA from fibroblasts from donor 198VI |
| GSM4988104 |
genomic DNA from fibroblasts from donor CS333VI |
| GSM4988105 |
genomic DNA from fibroblasts from donor CS466VI |
| GSM4988106 |
genomic DNA from fibroblasts from donor CS539VI |
| GSM4988107 |
genomic DNA from fibroblasts from donor CS548VI |
| GSM4988108 |
genomic DNA from fibroblasts from donor UVSS1VI |
| GSM4988109 |
genomic DNA from fibroblasts from donor CS797VI |
| GSM4988110 |
genomic DNA from fibroblasts from donor CS816VI |
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| This SubSeries is part of SuperSeries: |
| GSE163841 |
DNA methylation of fibroblasts from Cockayne syndrome and UV-sensitivity syndrome patients |
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| Relations |
| BioProject |
PRJNA687744 |
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