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| Status |
Public on Dec 11, 2020 |
| Title |
SLUG is enhanced by chemotherapeutics and functions to promote invasion and metastasis by directly targeting MMP3 in Cervical Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Neoadjuvant chemotherapy (NACT) followed by radical surgery for patients in FIGO stages IB2-IIB has proven to reduce tumor volume and numbers. However, NACT followed by radical surgery has not been found to improve overall survival. In this study, 35 paired pre/post-NACT cervical cancer tissues and 167 cervical cancer samples were examined by immunohistochemical analysis, SLUG expression was potentially increased in cervical cancer tissues after neoadjuvant chemotherapy, which in turn positively correlated with pelvic lymph node metastasis. The expression of SLUG following neoadjuvant chemotherapy was identified as a poor survival indicator. Functional assays, Agilent microarray hybridization and CHIP assay were performed to determine SLUG’s downstream genes and pathways. SLUG overexpression promoted metastasis in cervical cancer in both in vitro and in vivo models, whereas SLUG silencing had the opposite effect. Mechanically, SLUG is not a prerequisite for EMT activation in cervical cancer models, but contributes to the invasiveness and metastasis of cervical cancer cells by directly tethering to the recognition sequence GCCAGGTGC in the MMP3 promoter region. Our results provide mechanistic insight into the potential pro-cancer effect of neoadjuvant chemotherapy, and demonstrates that the SLUG-MMP3 axis could serve as a target for improving the efficacy of chemotherapy.
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| Overall design |
Using a Aligent SurePrint G3 Human Gene Expression v3 8×60K Microarray, to found genes changed significantly in SLUG overexpressing Siha cells compared with control Siha cells
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| Contributor(s) |
Jiang S, Zhang L |
| Citation missing |
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| Submission date |
Dec 10, 2020 |
| Last update date |
Dec 13, 2020 |
| Contact name |
Senwei Jiang |
| E-mail(s) |
jiangsw3@mail3.sysu.edu.cn
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| Phone |
18620779667
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| Organization name |
Sun Yat-sen University Cancer Center
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| Street address |
651 dongfengdong road
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| City |
GUANGZHOU |
| State/province |
GUANGDONG |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA684312 |
