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| Status |
Public on Dec 15, 2020 |
| Title |
Transcriptional effect of early-life adversity in adult hippocampus |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Early-life adversity (ELA) is associated with lifelong memory deficits, yet the responsible mechanisms remain unclear. We imposed ELA by rearing rat pups in simulated poverty, assessed hippocampal memory, and probed changes in gene expression, their transcriptional regulation and the consequent changes in hippocampal neuronal structure. ELA rats had poor hippocampal memory and stunted hippocampal pyramidal neurons, associated with ~140 differentially expressed genes. Upstream regulators of the altered genes included glucocorticoid receptor and, unexpectedly, the transcription factor neuron-restrictive silencer factor (NRSF/REST). NRSF contributed critically to the memory deficits because blocking its function transiently following ELA rescued spatial memory and restored the dendritic arborization of hippocampal pyramidal neurons in ELA rats. Blocking NRSF function in vitro augmented dendritic complexity of developing hippocampal neurons, suggesting that NRSF represses genes involved in neuronal maturation. These findings establish important, surprising contributions of NRSF to ELA-induced transcriptional programming that disrupts hippocampal maturation and memory function.
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| Overall design |
Gene expression was profiled in dorsal hippocampi from 8-week-old male rats that were subjected to either control rearing conditions (n=4) or ELA (n=5), and did not undergo behavioral testing.
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| Contributor(s) |
Schulmann A, Bolton JL, Regev L, Jiang S, Chen Y, Molet J, Garcia-Curran MM, Singh-Taylor A, Kamei N, Shao M, Noam Y, Mortazavi A, Baram TZ |
| Citation(s) |
33326786 |
| Submission date |
Nov 15, 2020 |
| Last update date |
Jan 26, 2021 |
| Contact name |
Anton Schulmann |
| E-mail(s) |
anton.schulmann@nih.gov
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| Organization name |
NIH/NIMH
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| Lab |
McMahon
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| Street address |
35 Convent Dr
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| City |
Bethesda |
| State/province |
Maryland (MD) |
| ZIP/Postal code |
20814 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA678522 |
| SRA |
SRP292611 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE161498_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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