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| Status |
Public on Oct 30, 2023 |
| Title |
Zebrafish as an animal model for the antiviral RNA interference pathway |
| Organism |
Danio rerio |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We have evaluated the possible use of zebrafish to study antiviral RNAi with sindbis virus (SINV), vesicular stomatitis virus (VSV), and nodamura virus (NoV). We find that SINV and NoV viruses induce the production of virus-derived small interfering RNAs (vsiRNAs), the hallmark of antiviral RNAi, with a preference of 22 nucleotides in length after infection of larval zebrafish. Meanwhile, the suppressor of RNAi (VSR) protein, NoV B2, may affect the accumulation of the NoV virus in zebrafish.
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| Overall design |
5 virus-derived small RNA from zebrafish infected with different viruses was detected by small RNA-seq.
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| Contributor(s) |
Ren Y, Li X, Tian Z, Xu Y, Zhang R, Li Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 30, 2020 |
| Last update date |
Oct 30, 2023 |
| Contact name |
Yang Li |
| E-mail(s) |
yangli15@fudan.edu.cn
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| Organization name |
Fudan University
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| Street address |
2005 Songhu Road
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (2) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA673345 |
| SRA |
SRP290217 |
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