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| Status |
Public on May 19, 2021 |
| Title |
Gene expression data from human circulating myeloid-derived suppressor cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
αβT cell- and B cell-depleted HLA-haploidentical haematopoietic stem cell transplantation is a life-saving therapeutic option to treat patients with high-risk leukemia. The G-CSF treatment stimulates mobilization from the bone marrow to blood of hematopoietic stem cells (HSC), and this manipulated graft also contains mature donor-derived NK and γδT cells, both exerting graft-versus-leukemia activity and control of infections at early stages after transplantation. The G-CSF-induced mobilization in the donor causes relevant increases of different myeloid cells, including polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC). PMN-MDSC are present in high proportions in the graft and exert a sharp inhibition on the effector functions of co-infused mature NK cells. Conversely, low frequencies of PMN-MDSCs are detected in the blood of non-mobilized healthy donors.
We used microarray technology to identify possible differences in the transcriptional programme of PMN-MDSCs isolated from blood of G-CSF mobilized donors as compared to those of non-mobilized healthy individuals.
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| Overall design |
PMN-MDSC were purified after density gradient centrifugation and immune-capture with CD66b beads from blood of 8 mobilized donors upon G-CSF treatment and from 5 non-mobilized donors as control group. Cell purity was confirmed by flow cytometry. After RNA extraction, samples were hybridized on Affymetrix GeneChip microarray.
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| Contributor(s) |
Pelosi A, Tumino N, Besi F, Vacca P, Moretta L |
| Citation(s) |
33986748 |
| Submission date |
Oct 06, 2020 |
| Last update date |
May 19, 2021 |
| Contact name |
Andrea Pelosi |
| E-mail(s) |
andrea.pelosi@opbg.net, pelosi.andrea81@gmail.com
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| Phone |
+390668594418
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| Organization name |
Ospedale Pediatrico Bambino Gesù
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| Department |
Laboratories
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| Lab |
Immunology
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| Street address |
Viale di San Paolo, 15
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| City |
Rome |
| State/province |
RM |
| ZIP/Postal code |
00146 |
| Country |
Italy |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA667604 |
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