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| Status |
Public on Oct 01, 2020 |
| Title |
Lung transcriptome sequencing anlysis in Male Sprague-Dawley rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Lung transcriptome sequencing anlysis in LPS-induced ALI model rats (Male Sprague-Dawley rats) after treatment with Ma Xing Shi Gan Decoction, In summary, our study suggests that MXSG inhibits viral invasion, proliferation, and mitigation of virus-induced lung injury, which may be a key mechanism of its therapeutic effect on COVID-19. These results provide experience for the treatment of infectious diseases and lung injury.
we dissected the chemical components of MXSG by liquid chromatography-mass spectrometry (LC-ESI-MS/MS) and analyzed the intervention pathways of MXSG based on components detected through network pharmacology. At the same time, the therapeutic effect of MXSG on COVID-19 was explained through published articles, and the relevant regulatory mechanism was proposed. Then, in this study, the regulatory effect of MXSG on inflammatory lung injury was explorated through transcriptome results.
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| Overall design |
Eight or nine-week-old Male SD rats (110±10 g) were maintained under specific pathogen-free conditions according to agency guidelines. The rats were kept with a 12-h light/dark cycle and with access to water and food ad libitum. After three days of adaptive breeding, 30 rats were randomly divided into three groups (n=10): normal group, pneumonia model group, Ma Xing Shi Gan Decoction (MXSG) group.
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| Contributor(s) |
li q, bai c |
| Citation(s) |
33324213 |
| Submission date |
Sep 30, 2020 |
| Last update date |
Dec 22, 2020 |
| Contact name |
qianqian li |
| E-mail(s) |
lqq@bucm.edu.cn
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| Organization name |
Beijing University of Chinese Medicine
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| Department |
School of Traditional Chinese Medicine
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| Street address |
No. 11, Bei San Huan Dong Lu, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
1000029 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA666668 |
| SRA |
SRP285932 |
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