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| Status |
Public on Apr 14, 2010 |
| Title |
Transcriptional networks regulated by drugs of abuse in mouse striatum |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In summary, we characterized genomic signatures of response to drugs of abuse and we found positive correlations between the drug-induced expression and various behavioral effects. These signatures are formed by two dynamically inducible transcriptional networks: (1) CREB/SRF-dependent gene pattern that appears to be related to drug-induced neuronal activity, (2) the pattern of genes controlled at least in part via release of glucocorticoids and androgens that are associated with rewarding and harmful drug effects. The discovery of co-expressed networks of genes allowed for the identification of master-switch controlling factors involved in molecular response to the drugs. Finally, using the pharmacological tools we were able to dissect and inhibit particular gene expression patterns from genomic profile.
Type: Drug response, Time-course, Gene expression profiling with Illumina Microarrays
Keywords: Addiction, Drugs of abuse, Time-course, Immediate Early Genes, Glucocorticoid receptor dependent genes, Cocaine, Heroin, Nicotine, Ethanol, Morphine, Methamphetamine
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| Overall design |
The microarray experiment was performed to analyze time-course of drug-induced transcriptional response in C57BL/6J mouse striatum. Six the most addictive and harming drugs of abuse (morphine 20 mg/kg, heroin 10 mg/kg, ethanol 2 g/kg, nicotine 1 mg/kg, methamphetamine 2 mg/kg or cocaine 25 mg/kg, i.p.) were selected for the comparison. Drug doses were previously reported as rewarding in mice and further tested in our laboratory. To analyze dynamics of early, intermediate and relatively late changes of mRNA abundance the experiment was performed in four time points (1, 2, 4 and 8h after drug administration). To exclude influence of drug injection and circadian rhythm on gene expression profile, control groups of saline treated and naïve animals were prepared for each time point. Design of the experiment assumed pooling of two animals per each array and using of three independent arrays per group. To provide appropriate balance in the whole dataset groups were equally divided between the array hybridization batches.
'Complete' normalized data and non-normalized data (containing control rows not represented in Platform GPL6105) are linked below as supplementary files.
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| Contributor(s) |
Piechota M, Korostynski M |
| Citation(s) |
20459597, 24010892 |
| Submission date |
Apr 22, 2009 |
| Last update date |
Sep 28, 2015 |
| Contact name |
Marcin Piechota |
| E-mail(s) |
marpiech@if-pan.krakow.pl
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| Phone |
(+4812) 6623328
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| Organization name |
Institute of Pharmacology PAS
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| Department |
Molecular Neuropharmacology
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| Street address |
Smetna 12
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| City |
Krakow |
| ZIP/Postal code |
31-343 |
| Country |
Poland |
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| Platforms (1) |
| GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
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| Samples (108)
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| Relations |
| BioProject |
PRJNA116841 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15774_RAW.tar |
5.2 Mb |
(http)(custom) |
TAR |
| GSE15774_matrix_non-normalized_complete.txt.gz |
30.7 Mb |
(ftp)(http) |
TXT |
| GSE15774_matrix_normalized_complete.txt.gz |
28.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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