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| Status |
Public on Mar 22, 2021 |
| Title |
Identification of gene modulating vascular inflammation using chromosome 2 fragment substitutions and RNA sequencing [miRNA-Seq] |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Chromosome 2 introgression from normotensive Brown Norway (BN) rats into hypertensive Dahl salt-sensitive (SS) background (consomic S2B) reduced blood pressure (BP) and vascular inflammation under normal salt diet (NSD). We hypothesized that BN chromosome 2 contains anti-inflammatory genes that could reduce BP elevation and vascular inflammation in rats fed NSD and high salt diet (HSD). We used chromosome 2 fragment substitutions to map chromosome 2 portion associated with vascular inflammation changes and next generation sequencing (NGS) to profile microRNAs in thoracic descending aorta of SS and congenic rats fed NSD or HSD.
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| Overall design |
Four- to 6-week-old male SS and congenic rats containing the Brown Norway (BN) chromosome (Chr) 2 distal portion (identified as SBA or S2Ba) and middle segment (identified as SBB or S2Bb) were fed NSD or HSD (4% NaCl) up to 12-to-13 weeks old. At the end of the treatment period, total RNA was extracted from a segment of the thoracic descending aorta and microRNAs were profiled by small RNA sequencing using Illumina HiSeq 2500.
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| Contributor(s) |
Berillo O, Ouerd S, Idris-Khodja N, Rehman A, Richer C, Sinnett D, Kwitek AE, Paradis P, Schiffrin EL |
| Citation(s) |
33161775 |
| Submission date |
Apr 28, 2020 |
| Last update date |
Mar 23, 2021 |
| Contact name |
Pierre Paradis |
| E-mail(s) |
pierre.paradis@mcgill.ca
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| Phone |
5143408222
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| Organization name |
Lady Davis Institute for medical research, Jewish General Hospital, McGill University
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| Street address |
3755 ch. de la Côte-Sainte-Catherine
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| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3T 1E2 |
| Country |
Canada |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE149516 |
Identification of gene modulating vascular inflammation using chromosome 2 fragment substitutions and RNA sequencing |
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| Relations |
| BioProject |
PRJNA628973 |
| SRA |
SRP258940 |
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