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| Status |
Public on Dec 31, 2022 |
| Title |
Lung response to multi-walled carbon nanotube exposure in rats. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The projected increase in the production and use of nanomaterials is expected to result in a corresponding increase in human exposure, potentially resulting in significant morbidity and mortality. Currently, the lung toxicity of multi-walled carbon nanotubes (MWCNT), a prototype nanomaterial, was investigated in a rat model. The rats were exposed by whole-body inhalation to air (controls) or MWCNT (6 hours/day, 3 days) to result in cumulative doses of 180, 90, 45, 22.5, or 11.25 mg/m3. Lung toxicity and gene expression profiles were determined in the lungs of the control and MWCNT exposed rats one day following termination of the exposures. Moderate lung histological changes, indicative of toxicity, were detected in rats exposed to MWCNT at doses > 45 mg/m3. Changes in toxicity parameters including, lactate dehydrogenase (LDH) activity, oxidant production, cell counts of phagocytes, and cytokine levels were detected in the bronchoalveolar lavage of rats exposed to > 22.5 mg/m3 MWCNT, compared with the air controls. Lung gene expression profiling detected differences in transcript levels (fold change >1.5 and FDR p<0.05) of several genes in the rats exposed to >22.5 mg/m3 MWCNT, compared with the controls. The changes in lung toxicity and gene expression profiles exhibited a dose-response to the MWCNT administered in the rats.
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| Overall design |
Approximately 3 months old male Fischer rats (Charles River Laboratories, Wilmington, MA) were used in this study, conducted in an AAALAC International approved animal facility (NIOSH, Morgantown, WV) following a protocol approved by the NIOSH Animal Care and Use Committee. Rats were housed in groups of 3 rats/cage. The rats (n=6) were exposed by whole-body inhalation to MWCNT (6 hours/day, 3 days) to result in cumulative doses of 180, 90, 45, 22.5, or 11.25 mg/m3. Separate groups of rats (n=6) exposed simultaneously to filtered air served as the controls. One day following termination of exposure to air or multi-walled carbon nanotubes, the rats were euthanized, and lung gene expression profiles determined by RNA sequencing.
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| Contributor(s) |
Sager TM, Umbright CM, Joseph P |
| Citation(s) |
35648795 |
| Submission date |
Apr 17, 2020 |
| Last update date |
Feb 10, 2023 |
| Contact name |
Christina M Umbright |
| Organization name |
DHHS/PHS/CDC/NIOSH
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| Department |
HELD
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| Lab |
L-4324
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| Street address |
1095 Willowdale Road
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| City |
Morgantown |
| State/province |
WV |
| ZIP/Postal code |
26505 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA625993 |
| SRA |
SRP256969 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148869_MW_Lung_hisatCounts_All.csv.gz |
1.7 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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