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| Status |
Public on Aug 01, 2023 |
| Title |
Micro RNA Expression data from DU145 shDCUN1D1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Defective in Cullin Neddylation 1 Domain Containing 1 (DCUN1D1) is an E3 ligase for neddylation, a post translational process similar to, and that runs in parallel with the ubiquitin proteasome pathway. Although being established as an oncogene in various squamous cell carcinomas, the role of DCUN1D1 in prostate cancer has not been explored. Here we used microarray analysis to determine the set of genes regulated by DCUN1D1 in prostate cancer cells. Using the prostate cancer DU145 cell line as model, and a lentivirus encoding shRNA-DCUN1D1, we generated DCUN1D1 knockdown cell lines. Microarray analysis identified deregulation of key gene expression, cellular growth and proliferation, developmental, cell death and cancer pathways as possible mechanisms mediated by DCUN1D1 in prostate cancer.
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| Overall design |
Total RNA was isolated from DU145 stabley transduced with lentivirus expresssing shRNA against DCUN1D1 or GFP (control) and hybridized to HT Human U133 gene .
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| Contributor(s) |
Bashin M, Libermann T, Gu X |
| Citation(s) |
37566052 |
| Submission date |
Mar 31, 2020 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Luiz F Zerbini |
| E-mail(s) |
luiz.zerbini@icgeb.org
|
| Organization name |
ICGEB
|
| Lab |
Cancer Genomics
|
| Street address |
Wernher and Beit Building (South), Anzio Road
|
| City |
Cape Town |
| ZIP/Postal code |
7925 |
| Country |
South Africa |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA621367 |
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