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| Status |
Public on Feb 10, 2009 |
| Title |
Expression data from Drosophila histone methyltransferase mutants |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
H3K9 methylation is a mark of inactive chromatin. In D.melanogaster, three enzymes (SU(VAR)3-9, EGG, and dG9A) are responsible for creating the H3K9 methyl mark. We have investigated the effects of loss of SU(VAR)3-9 and EGG on gene expression in adult heads using microarray analysis.
Keywords: Expression profiling by microarray
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| Overall design |
Heads from adults flies were used for RNA extraction and hybridization on Affymetrix microarrays. Four genotypes were used: yw; +/CyO, yw; egg235/CyO, yw; +/CyO; Su(var)3-906; yw; egg235/CyO; Su(var)3-906. For each genotype, three biological replicates were analyzed. For the yw; +/CyO control, an additional technical replicate (same RNA sample processed twice) is included. While source files for both technical replicates are included, only one was used for the final analysis.
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| Contributor(s) |
Riddle NC |
| Citation(s) |
19189944 |
| Submission date |
Feb 09, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nicole Christina Riddle |
| Organization name |
Washington University
|
| Department |
Biology
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| Lab |
SCR Elgin
|
| Street address |
1 Brookings Dr.; CB1137
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63130 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA112251 |
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