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| Status |
Public on Aug 10, 2020 |
| Title |
RNAseq of NRVMs infected with AdCryABR120G by the manipulation of Ube2v1 expression |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Neonatal rat ventricle myocytes (NRVMs) were infected with CryABR120G adenovirus. Knockdown or overexpression of Ube2v1 was performed by siUbe2v1 transfection or AdUbe2v1 infection in CryABR120G-expressing cells. Total RNA was extracted from NRVMs of four groups: CTR (untreated control), R120G (AdCryABR120G infection), RSIV1 (AdCryABR120G infection with siUbe2v1 transfection), and RADV1 (coinfection with AdCryABR120G and AdUbe2v1), in triplicate, for gene expression profiling.
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| |
|
| Overall design |
Total RNA from NRVMs in triplicate per group was pooled for deep RNA sequencing
|
| |
|
| Contributor(s) |
Xu N, Robbins J, Yutzey KE |
| Citation(s) |
32081062 |
| Submission date |
Feb 07, 2020 |
| Last update date |
Nov 09, 2020 |
| Contact name |
Katherine E Yutzey |
| E-mail(s) |
katherine.yutzey@cchmc.org
|
| Phone |
513-636-8340
|
| Organization name |
Cinicnnati Children's Hospital Medical Center
|
| Department |
Divison of Molecular Cardiovascular Biology
|
| Street address |
240 Albert Sabin Way
|
| City |
Cinicnnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA605478 |
| SRA |
SRP247680 |
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