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Series GSE143439 Query DataSets for GSE143439
Status Public on May 07, 2020
Title Nanog affects establishment of chromatin marks at zebrafish Zygotic Genome Activation
Organism Danio rerio
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In zebrafish embryos, the maternal transcription factors (TFs)Nanog, Pou5f3 and Sox19b activate the early zygotic gene expression. To evaluate the role of Nanog, we examined the changes in H3K27ac and H3K4me3 chromatin modifications in the MZnanog embryos mutant by maternal and zygotic Nanog.
 
Overall design We performed ChIP-seq for H3K27ac and H3K4me3 chromatin modifications in the embryos mutant by Nanog transcription factor (MZnanog). Experiments were performed at dome stage (4.3 hours post-fertilization (hpf).
 
Contributor(s) Gebhard A, Onichtchouk D
Citation(s) 37709752
Submission date Jan 10, 2020
Last update date Sep 21, 2023
Contact name Daria Onichtchouk
E-mail(s) daria.onichtchouk@biologie.uni-freiburg.de
Phone 00497612032595
Organization name University of Freiburg
Street address Hauptstrasse 1
City Freiburg
ZIP/Postal code 79104
Country Germany
 
Platforms (1)
GPL14875 Illumina HiSeq 2000 (Danio rerio)
Samples (4)
GSM4259502 MZnanog_H3K4me3_ChIP-seq
GSM4259503 MZnanog_input_for_H3K4me3
GSM4259504 MZnanog_H3K27ac_rep1_ChIP-seq
Relations
BioProject PRJNA600508
SRA SRP241206

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143439_RAW.tar 1.2 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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