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Series GSE142478 Query DataSets for GSE142478
Status Public on Jul 13, 2020
Title Sex- and region-specific transcriptomic differences in brainstem and cervical spinal cord microglia in rat offspring exposed to gestational intermittent hypoxia 
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary We analyzed gene expression via RNA-sequencing in medulla and C3-C6 cervical spinal cord microglia isolated from young adult rats exposed to gestational intermittent normoxia or gestational intermittent hypoxia
 
Overall design G10 sprague dawley rat dams were exposed to one of the following conditions: 1) 11 days of intermittent normoxia (21%/21% O2) or 2) 11 days of intermittent hypoxia (21%/10.5% O2). Offspring were born under normoxic conditions and allowed to grow to 8 weeks. Medulla and C3-C6 cervical spinal cord from 1 male and 1 female from 5 paired litters were harvested. Microglia were immunomagnetically isolated from each region and total RNA isolated for sequencing.
 
Contributor(s) Watters JJ, Ewald AC
Citation(s) 32661056
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HL142752 Fetal reprogramming by gestational intermittent hypoxia impairs respiratory neuromotor control in adult offspring UNIVERSITY OF WISCONSIN MADISON Tracy L Baker
R01 HL142752 Fetal reprogramming by gestational intermittent hypoxia impairs respiratory neuromotor control in adult offspring UNIVERSITY OF WISCONSIN MADISON JYOTI J WATTERS
F31 NS100229 The Role of TLR4 signaling and histone modifications in neonatal microglial priming by chronic intermittent hypoxia UNIVERSITY OF WISCONSIN MADISON Elizabeth Anne Kiernan
Submission date Dec 20, 2019
Last update date Dec 16, 2020
Contact name Andrea C Ewald
Organization name University of Wisconsin - Madison
Department Comparative Biomedical Sciences
Lab Watters
Street address 2015 Linden Dr
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (40)
GSM4230169 male normoxia medulla replicate 1 [MNV_B1]
GSM4230170 male normoxia medulla replicate 2 [MNV_B2]
GSM4230171 male normoxia medulla replicate 3 [MNV_B3]
Relations
BioProject PRJNA597110
SRA SRP238460

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Supplementary file Size Download File type/resource
GSE142478_all_BS_SC_readcount.txt.gz 1.2 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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