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| Status |
Public on Apr 04, 2020 |
| Title |
Tuberculosis-associated IFN-I induces Siglec-1 on microtubule-containing tunneling nanotubes and favors HIV-1 spread in macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To understand the susceptbility of human M(IL-10) macrophages to human immunodeficiency virus, we performed a genome-wide transcriptome analysis in these cells to identify a gene profile responsible for this phenomenon. A distinct 60 gene-transcript signature was defined in M(IL-10) macrophages, using a combination of the expression level, statistical filters and hierarchical clustering; 51 genes were up-regulated and 9 genes were down-regulated in M(IL-10) macrophages compared to control cells. Among the genes upregulated, we identified a interferon-stimulated gene (ISG) signature include SIGLEC-1. We confirmed SIGLEC-1 and other five ISG genes by qPCR, FACS and western blot.
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| Overall design |
Human macrophages were diffentiated from freshly isolated monocytes treated with M-CSF and conditioned media. Conditioned media was generated from Mycobacterium tuberculosis-infected (coMtb) or non-infected (coCtrl) macrophages. Upon confirmation of the M(IL-10) macrophage phenotype induced by coMtb, and susceptbility to HIV-1 infectiion, the transcriptome analyses was performed in these conditioned cells from four independent donors.
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| Contributor(s) |
Souriant S, Cougoule C, Mercier I, Dupont M, Vu Manh T, Vérollet C, Lugo-Villarino G |
| Citation(s) |
32223897 |
| Submission date |
Oct 28, 2019 |
| Last update date |
Apr 14, 2020 |
| Contact name |
Thien-Phong Vu Manh |
| Organization name |
CIML
|
| Street address |
163 avenue de Luminy
|
| City |
marseille |
| ZIP/Postal code |
13288 |
| Country |
France |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA580006 |
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