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| Status |
Public on Dec 16, 2021 |
| Title |
RNAseq analysis of retinal microglia during early diabetes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the use of RNAseq to identify gene changes in isolated retinal microglia after 4 weeks of STZ-induced diabetes. At this early time point in diabetes induction, single retinal cell suspensions were sorted via FACS using a Cd11b-FITC conjugate. RNA was isolated and preamplified using the SMARTSeq v4 low RNA input kit. After library preparation a 50bp single end read was performed at a depth of 19-34 million reads per sample using the Illumina HiSeq. Constructs were mapped to the rat genome and differential expression calculated between the STZ-treated and control groups.
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| |
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| Overall design |
Examination of 2 groups (control and diabetic) after 4 weeks of STZ induced diabetes
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| |
|
| Contributor(s) |
Jobling AI, Fletcher EL |
| Citation(s) |
34903661 |
| Submission date |
Oct 23, 2019 |
| Last update date |
Dec 16, 2021 |
| Contact name |
Andrew Ian Jobling |
| E-mail(s) |
aij@unimelb.edu.au
|
| Organization name |
University of Melbourne
|
| Department |
Anatomy & Neuroscience
|
| Street address |
Medical Building, Cnr Grattan St and Royal Pde
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3010 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA579063 |
| SRA |
SRP226697 |
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