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| Status |
Public on Aug 20, 2022 |
| Title |
Exploring the molecular mechanism and and related signaling pathways of annexin A2 in human retinal vascular endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the role and mechanism of ANXA2 in retinal neovascularization, we used interfering and overexpressing lentivirus to intervene in HREC cells, respectively. Divided into interference group (negative control group and positive interference group), overexpression group (negative control group, overexpression positive control group). The mechanism of action of annexin A2 in human retinal vascular endothelium was explored by real-time PCR to quantify the expression of molecular genes in samples.
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| Overall design |
We used interfering and overexpressing lentivirus to intervene in HREC cells for 72 hours and isolated samples for gene expression. Three independent experiments were performed for each sample.
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| Contributor(s) |
Zhao ZC, Guo T, Zhao SH |
| Citation missing |
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| Submission date |
Aug 21, 2019 |
| Last update date |
Aug 22, 2022 |
| Contact name |
zichang zhao |
| Organization name |
Changhai Hospital
|
| Department |
Ophthalmology
|
| Street address |
168 changhai road
|
| City |
shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (12)
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| GSM4041358 |
HREC-interfering_negative control group [Sh-NC-1] |
| GSM4041359 |
HREC-interfering_negative control group [Sh-NC-2] |
| GSM4041360 |
HREC-interfering_negative control group [Sh-NC-3] |
| GSM4041361 |
HREC-interfering_positive interference group [Sh-A2-1] |
| GSM4041362 |
HREC-interfering_positive interference group [Sh-A2-2] |
| GSM4041363 |
HREC-interfering_positive interference group [Sh-A2-3] |
| GSM4041364 |
HREC-overexpressing_negative control group [Lenti-A2-1] |
| GSM4041365 |
HREC-overexpressing_negative control group [Lenti-A2-2] |
| GSM4041366 |
HREC-overexpressing_negative control group [Lenti-A2-3] |
| GSM4041367 |
HREC-overexpressing_overexpression positive control group [Lenti-EGIP-1] |
| GSM4041368 |
HREC-overexpressing_overexpression positive control group [Lenti-EGIP-2] |
| GSM4041369 |
HREC-overexpressing_overexpression positive control group [Lenti-EGIP-3] |
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| Relations |
| BioProject |
PRJNA561341 |
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