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| Status |
Public on Jun 21, 2019 |
| Title |
Progression of liver tumor was promoted by tris(1,3-dichloro-2-propyl)phosphate through the induction of inflammatory responses in krasV12 transgenic zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, krasV12 genetically modified zebrafish, Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasG12V), an inducible liver tumor model, was used to evaluate the promotion potential of TDCIPP for liver tumor. Briefly, krasV12 transgenic females were exposed to 0.3 mg/L TDCIPP, 20 mg/L doxycycline (DOX) and a binary mixture of 0.3 mg/L TDCIPP with 20 mg/L DOX, and liver size, histopathology, and transcriptional profiles of liver were tested. Treatment with TDCIPP increased the liver size and caused more aggressive hepatocellular carcinoma (HCC). Furthermore, compared with the exposure to DOX, TDCIPP in the presence of DOX up-regulated the expression of genes relevant with salmonella infection and the toll-like receptor signaling pathway, implying an occurrence of inflammatory reaction, which was sustained by the increase in the amount of infiltrated neutrophils in the liver of Tg(lyz:DsRed2) transgenic zebrafish larvae. Collectively, our results suggested that TDCIPP could promote the liver tumor progression by induction of hepatic inflammatory responses.
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| Overall design |
Liver mRNA prolifie of 3 months old kras zebrafish which were treated with DMSO, TDCIPP, DOX, DOX with TDCIPP were generated by deep sequencing, in triplicate.
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| Contributor(s) |
Chen S, Liu C |
| Citation missing |
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| Submission date |
Jun 20, 2019 |
| Last update date |
Jun 21, 2019 |
| Contact name |
Chen Sheng |
| E-mail(s) |
cs55490964@163.com
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| Phone |
17607175732
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| Organization name |
Novogene
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| Street address |
Wuqing
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| City |
Tianjing |
| ZIP/Postal code |
301700 |
| Country |
China |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA549880 |
| SRA |
SRP201987 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133076_Processed_data.txt.gz |
12.7 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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