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| Status |
Public on Nov 22, 2019 |
| Title |
The MicroRNAs target mitochondrial dynamics and metabolism to regulate mitochondrial-mediated antiviral innate immunity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNAs (miRNAs) are short non-coding RNAs act as a suppressor of multiple genes and it is known that miRNAs link to numerous biological functions. Mitochondria act as a platform for innate immune response against RNA viruses via a mitochondrial outer membrane protein, MAVS. Here we report miR-302b and miR-372 involve in mitochondrial-mediated antiviral response. These miRNAs were upregulated by viral infection and influenced not only the production of type I interferon and inflammatory cytokines, but also mitochondrial function. Furthermore, we revealed that these phenotypes were induced by changing several mitochondrial genes expression. miR-302b induced mitochondrial fragmentation by changing gene expression related to mitochondrial dynamics to regulate Drp1 activity. Indeed, downregulation of SLC25A12 by miR-302b suppressed antiviral responses via inhibiting the MAVS activation directly. We first propose that miR-302b and miR-372 act as negative regulator of antiviral response by affecting mitochondrial function to inhibit excessive innate immune response.
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| Overall design |
Change of gene expression by miR-302b in HEK293 cells was measured. The gene expression in the cells transfected with miR-302b mimic was compared with the cells transfected with negative control miRNA mimic.
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| Contributor(s) |
Yasukawa K, Koshiba T |
| Citation(s) |
31767682 |
| Submission date |
Apr 10, 2019 |
| Last update date |
Jan 13, 2020 |
| Contact name |
Kai Yasukawa |
| Organization name |
Kyusyu University
|
| Department |
Department of Systems Life Sciences
|
| Street address |
744 Motooka, Nishi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
819-0395 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA531936 |
