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| Status |
Public on Jun 01, 2019 |
| Title |
Gene expression analysis of human CD34+ AML cells cultured with or without gabapentin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To clarify the global gene expression alteration in primary CD34+ AML cells after BCAA metabolism inhibition, CD34+ AML cells were treated with or without gabapentin in vitro. After 9-12 hours of culture with or without gabapentin, FACS-purified CD34+ AML cells were subjected to transcriptome analysis.
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| Overall design |
To clarify how BCAA metabolism affect the gene expression of human CD34+ AML cells, CD34+ AML cells from 4 independent patients were treated with or without 40mM gabapentin, a specific BCAT1 inhibitor for 9-12 hours. After BCAA metabolism inhibition by gabapentin, CD34+ AML cells were purified and total RNA was extracted from control and treated samples.
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| Contributor(s) |
Kikushige Y, Akashi K |
| Citation(s) |
36044390 |
| Submission date |
Feb 26, 2019 |
| Last update date |
Sep 22, 2022 |
| Contact name |
Yoshikane Kikushige |
| E-mail(s) |
kikusige@intmed1.med.kyushu-u.ac.jp
|
| Organization name |
Kyushu University
|
| Street address |
3-1-1 Maidashi, Higashiku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA524297 |
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