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| Status |
Public on Jul 12, 2019 |
| Title |
Neonatal intermittent hypoxia exposure sex-specifically augments rat microglial cytokine expression acutely and long-term |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed gene expression via RNA-sequencing in microglia isolated from postnatal day 9 rats exposed to normoxia or intermittent hypoxia, in the presence or absence of lipopolysaccharide
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| Overall design |
P1 sprague dawley rat neonates (three biological replicates per sex and condition) were exposed to one of the following conditions: 1) 8 days of normoxia (21 % O2 followed by 3 hours saline injection (i.p.) 2) 8 days of normoxia followed by 3 hours lipopolysaccharide (LPS) injection (0.1 mg/kg i.p.) 3) 8 days of intermittent hypoxia (2 min cycles between 10.5% O2 and 21% O2 8 hrs/day) followed by vehicle injection and 3) 8 days of intermittent hypoxia followed by 3 hrs LPS injection). Microglia were immunomagnetically isolated following the vehicle or LPS injection and total RNA isolated for sequencing.
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| Contributor(s) |
Watters JJ, Kiernan EA |
| Citation |
https://www.frontiersin.org/articles/10.3389/fimmu.2019.01479/full
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| Submission date |
Feb 22, 2019 |
| Last update date |
Jul 12, 2019 |
| Contact name |
Jyoti Watters |
| E-mail(s) |
jjwatters@wisc.edu
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| Phone |
6082656092
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| Organization name |
University of Wisconsin-Madison
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| Department |
CBS
|
| Lab |
Jyoti Watters
|
| Street address |
2015 Linden Drive
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA523827 |
| SRA |
SRP186609 |
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