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| Status |
Public on Feb 06, 2019 |
| Title |
α1ACT is essential for survival and early cerebellar programming in a critical neonatal window [ChIP-Seq] |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Previous study has shown that alpha1ACT is a transcription factor involved with regulating neuronal gene expression. We further performed ChIP-seq using pc12 cell lines stably expressing pcDNA3-alpha1ACT or control pcDNA3 vectors to identify genomic locus directly occupied by alpha1ACT
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| Overall design |
A total of 6 sequencing files collected from chromatin immunoprecipitation at day 3 were used for the ChIP-Seq study. Among them, 3 sequencing files were obtained from EV expressed tissues treated with M2 and 1804 antibody beads respectively, and corresponding ChIP-Seq input. The other 3 sequencing files corresponding to α1ACT expressed tissues treated with M2 and 1804 antibody and corresponding ChIP-Seq input.
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| Contributor(s) |
Du X, Gomez CM, We C, Parviz Hejazi Pastor D, Rao ER, Li Y, Grasselli G, Godfrey J, Palmenberg AC, Andrade J, Hansel C |
| Citation(s) |
30922876 |
| Submission date |
Feb 05, 2019 |
| Last update date |
May 08, 2019 |
| Contact name |
Yan Li |
| E-mail(s) |
yli22@bsd.uchicago.edu
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| Phone |
7738344316
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| Organization name |
The University of Chicago
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| Department |
Center for Research Informatics
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| Street address |
900 East 57th St.
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE126089 |
α1ACT is essential for survival and early cerebellar programming in a critical neonatal window |
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| Relations |
| BioProject |
PRJNA521004 |
| SRA |
SRP183694 |
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