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| Status |
Public on Nov 01, 2018 |
| Title |
Comprehensive and comparative analysis of gene expression induced by LPA- and CP-609,550 in normal human epidermal keratinocytes (NHEKs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We screened highly expressed G-protein coupled receptors (GPCRs) in normal human epidermal keratinocytes (NHEKs). We then tested the effect of ligands of selected GPCRs for their potency to induce the expression of FLG, a crucial skin barrier marker gene, in NHEKs. We used CP-609,550, a JAK inhibitor, as a positive control. As a result, we identified Oleoyl-L-α-lysophosphatidic acid (LPA) as a strong FLG inducer in NHEKs. In addition, we found that LPA induced NHEKs morphological changes characteristics of differentiated keratinocytes. Notably, although CP-609,550 also induced FLG expression in NHEKs, it didn't affect NHEKs cell morphology. We further conducted comprehensive and comparative microarray analysis of gene expression induced by LPA and CP-609,550 in NHEKs. We found that LPA treatment not only induced FLG expression but also expression of several genes related to keratinocytes differentiation, epidermis development and cornified envelope. This was not observed in the CP-609,550-treated cells.
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| Overall design |
Gene expression induced by 10μM Oleoyl-L-α-lysophosphatidic acid or 1μM CP-609,550 in NHEKs were measured at 72h and compared to gene expression in the vehicle control-treated NHEKs. Three independent experiments were performed for each condition.
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| Contributor(s) |
Siriwach R, Sumitomo A |
| Citation(s) |
30447238 |
| Submission date |
Oct 31, 2018 |
| Last update date |
Jan 31, 2019 |
| Contact name |
Ratklao Siriwach |
| E-mail(s) |
ratklao.siriwach@gmail.com
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| Organization name |
Kyoto University Graduate School of Medicine
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| Department |
Department of Drug Discovery Medicine
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| Street address |
Sakyo-ku, Shogoin Kawahara-cho
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| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA503179 |
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