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| Status |
Public on Oct 31, 2018 |
| Title |
The Lysosomal Transcription Factor TFEB regulates myelination downstream of the Rag-Ragulator complex. |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To identify genes that are differentially expressed in oligodendrocytes between control and rraga mutant zebrafish, we performed RNA seq using the Illumina platform
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| Overall design |
Oligodendrocytes were isolated from control and rraga mutant zebrafish at 5 days post fertilization using FACS sorting of Cldnk:GFP positive cells. RNA was extracted from these cells and sequenced using standard Illumina protocol.
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| Contributor(s) |
Meireles AM, Shen K, Iyer H, Talbot WS, Zoupi L, Williams A, Bouchard EL |
| Citation(s) |
30399334 |
| Submission date |
Aug 31, 2018 |
| Last update date |
Jan 30, 2019 |
| Contact name |
Harini Iyer |
| E-mail(s) |
iyerh@stanford.edu
|
| Phone |
6507257664
|
| Organization name |
Stanford University
|
| Department |
Developmental Biology
|
| Lab |
Talbot
|
| Street address |
279 Campus Drive, B300 Beckman Center
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA489469 |
| SRA |
SRP159668 |
