|
| Status |
Public on Jul 15, 2018 |
| Title |
Potential role of hoxb1b in zebrafish development |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
identification of differentially expressed genes in hoxb1b homozygous mutant hindbrain when compared to wildtype in zebrafish
|
| |
|
| Overall design |
Total RNA was extracted from pools of dechrionated, deyolked of hoxb1b homzygous mutants and wildtype embryos at 18hpf using the RNeasy Mini Kit (Qiagen). Three libraries from wildtype embryos and three libraries from hoxb1b mutants were then generated from 3mg RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina). All libraries were analyzed for quality on a bioanalyzer prior to sequencing (Agilent 2100 BioAnalyzer).
|
| |
|
| Contributor(s) |
Ghosh P, Sagerstrom CG |
| Citation(s) |
29945667 |
| Submission date |
Apr 19, 2018 |
| Last update date |
Jan 14, 2019 |
| Contact name |
Charles G Sagerstrom |
| E-mail(s) |
Charles.Sagerstrom@umassmed.edu
|
| Organization name |
UMass Medical School
|
| Department |
Biochemistry and Molecular Pharmacology
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (6)
|
| GSM3105079 |
wildtype control replicate 1 (hoxb1bmut) |
| GSM3105080 |
wildtype control replicate 2 (hoxb1bmut) |
| GSM3105081 |
wildtype control replicate 3 (hoxb1bmut) |
| GSM3105082 |
hoxb1b-/- mutant replicate 1 (hoxb1bmut) |
| GSM3105083 |
hoxb1b-/- mutant replicate 2 (hoxb1bmut) |
| GSM3105084 |
hoxb1b-/- mutant replicate 3 (hoxb1bmut) |
|
| This SubSeries is part of SuperSeries: |
| GSE113437 |
Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish |
|
| Relations |
| BioProject |
PRJNA451022 |
| SRA |
SRP141212 |
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