|
| Status |
Public on Jan 19, 2019 |
| Title |
RNA-seq of a high glucose induced oxidative stress and apoptosis model on primary rat cardiomyocytes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Diabetic hyperglycemia promotes reactive oxygen species (ROS) production to lead to oxidative stress and apoptosis responsible for progressive deterioration of the structure and function of organs. It has been indicated that factors and pathways regulating ROS production and the cellular redox state play a key role in the progression of diabetes and diabetes complications including cardiomyopathy. Recent studies had demonstrated that long non-coding RNAs (lncRNAs) played crucial roles on modulation of oxidative stress and apoptosis activity. In this study, we first established a high glucose induced oxidative stress and apoptosis model on primary rat cardiomyocytes. ROS formation and apoptosis activity were significantly increased at 24h/48h after high glucose stimulation. RNA sequencing analysis was applied to detect differentially expressed lncRNAs during cardiomyocytes oxidative stress and apoptosis.
|
| |
|
| Overall design |
RNA-seq of a high glucose induced oxidative stress and apoptosis model on primary rat cardiomyocytes and control groups at 24h/48h.
|
| |
|
| Contributor(s) |
Yu M, Zhao X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 19, 2018 |
| Last update date |
Jan 20, 2019 |
| Contact name |
manli Yu |
| Organization name |
Changhai Hospital of Shanghai
|
| Department |
Department of Cardiology
|
| Street address |
No.168 Changhai road
|
| City |
shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA430889 |
| SRA |
SRP131060 |
Less...
More...