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| Status |
Public on Jul 24, 2018 |
| Title |
Tdrd6a recruits Ziwi-bound piRNAs and coordinates deposition of germ plasm mRNAs into primordial germ cells [small RNA] |
| Organism |
Danio rerio |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Germ plasm, the Balbiani body and nuage are evolutionary conserved structures essential for germ cell specification and maintenance. We describe Tdrd6a as a component of these structures with two distinct molecular functions. First, Tdrd6a facilitates the accumulation of the typical antisense-bias of piRNAs, without having effects on piRNA biogenesis signatures. Second, we show that Tdrd6a is required for Balbiani body and germ plasm integrity, and associates with RNA-binding proteins and germ plasm mRNAs. On the cell-biological level, maternally contributed Tdrd6a strongly impacts germ cell formation, but is dispensable for fertility. Using single-cell RNA-sequencing we demonstrate that Tdrd6a promotes early germ cell development and regulates the stoichiometry of germ plasm mRNAs. We propose that Tdrd6a functions as a scaffold to recruit correct ratios of germ plasm transcripts and to accumulate antisense piRNA complexes in order to ensure both specification and maintenance of germ cells.
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| Overall design |
Zebrafish were grown under standard conditions. Total ovary or size selected Oocytes were used to extact RNA from het of tdrd6a mutant fish.
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| Contributor(s) |
Kaaij L |
| Citation(s) |
30086300 |
| Submission date |
Dec 04, 2017 |
| Last update date |
Jan 15, 2020 |
| Contact name |
lucas kaaij |
| E-mail(s) |
l.kaaij@imb-mainz.de
|
| Organization name |
IMB
|
| Department |
RNA silencing
|
| Lab |
Rene Ketting
|
| Street address |
ackermannweg 4
|
| City |
mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (6)
|
| GSM2875714 |
smRNA seq library of early oocytes from tdrd6a het fish |
| GSM2875715 |
smRNA seq library of late oocytes from tdrd6a mut fish |
| GSM2875716 |
smRNA seq library of early oocytes from tdrd6a mut fish |
| GSM2875717 |
smRNA seq library of late oocytes from tdrd6a hett fish |
| GSM2875718 |
smRNA seq library of ovary from tdrd6a het fish |
| GSM2875719 |
smRNA seq library of ovary from tdrd6a mut fish |
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| This SubSeries is part of SuperSeries: |
| GSE79285 |
Tdrd6a recruits Ziwi-bound piRNAs and coordinates deposition of germ plasm mRNAs into primordial germ cells |
|
| Relations |
| BioProject |
PRJNA421016 |
| SRA |
SRP126106 |
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