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| Status |
Public on Sep 17, 2018 |
| Title |
Dynamic remodeling of mRNA splicing and translation programs drives hepatocyte proliferation during liver regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During liver regeneration, most new hepatocytes arise from pre-existing ones; yet, the underlying mechanisms that drive these cells from quiescence to proliferation remain poorly defined. By using high-resolution transcriptome profiling of purified hepatocytes isolated from quiescent and toxin-injured adult mouse livers,we uncover an overlap of regenerative response with developmental transcriptome programming. The translational remodeling modulates protein levels ofa set of splicing factors including Epithelial splicing regulatory protein 2 (ESRP2), which activates an early postnatal splicing program in regenerating hepatocytes. We find that regeneration-regulated exons are enriched within unstructured regions of proteins and frequently harbor amino-acid residues that can be post-translationally modified to influence protein-protein interactions.
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| Overall design |
Hepatocytes were isolated from normal fed mice and DDC fed (4 week) mice. Additionally, hepatocytes were isolated from E18 livers wherein livers from approx. 8 E18 embryos were pooled to prepeare hepatocytes. From all samples total RNA was isolated and after polyA selection sequenced on an Illumina platform.
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| Contributor(s) |
Kalsotra A, Bangru S, Arif W |
| Citation(s) |
30250226 |
| Submission date |
Oct 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Sushant Bangru |
| E-mail(s) |
bangru2@illinois.edu, sushantbangru@gmail.com, sb725@duke.edu
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| Phone |
+1-217-979-9320
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| Organization name |
University of Illinois, Urbana-Champaign
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| Department |
Biochemistry
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| Lab |
Dr. Auinash Kalsotra
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| Street address |
600 S Mathews Avenue
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| City |
Urbana |
| State/province |
Illinois |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA415599 |
| SRA |
SRP121282 |
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