|
| Status |
Public on Feb 15, 2008 |
| Title |
Identification of genes controlled by LMX1B in the developing mouse hindlimb bud |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
A control vs. genetic knockout experiment aimed at determining what RNAs are upregulated or downregulated in e11.5 mouse proximal limb tissue lacking the Lmx1b gene. Because Lmx1b is required for dorsal-ventral patterning of the limb, this screen gives insight into what putative downstream targets of Lmx1b contribute to dorsal-ventral patterning.
Keywords: Genetic allele comparison
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| Overall design |
The proximal portion of e11.5 hindlimb buds (~500um) was used for RNA extraction. Each array was hybridized with pooled cRNAs from both hindlimb buds of three embryos (6 hindlimbs/sample).
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| |
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| Contributor(s) |
Krawchuk D, Kania A |
| Citation(s) |
18351676 |
| Submission date |
Feb 13, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
dayana krawchuk |
| E-mail(s) |
dayana.krawchuk@ircm.qc.ca
|
| Phone |
5149875588
|
| Organization name |
IRCM
|
| Street address |
110 Ave. des Pins O.
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H2W1R7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
| GSM264689 |
Wild-type E11.5 proximal hindlimb pool 1 |
| GSM264690 |
Wild-type E11.5 proximal hindlimb pool 2 |
| GSM264691 |
Wild-type E11.5 proximal hindlimb pool 3 |
| GSM264692 |
Lmx1b -/- E11.5 proximal hindlimb pool 1 |
| GSM264693 |
Lmx1b -/- E11.5 proximal hindlimb pool 2 |
| GSM264694 |
Lmx1b -/- E11.5 proximal hindlimb pool 3 |
|
| Relations |
| BioProject |
PRJNA107979 |
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