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| Status |
Public on Oct 01, 2021 |
| Title |
Understanding the role of miRNA-143 (miR-143) in ischemia-induced retinopathy via RNA transcriptome profiling |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate molecular network mediated by miR-143, we analyzed the transcriptome profile of retinas isolated from neonatal pups subjected to either normoxia or oxygen-induced retinopathy (OIR) for 14 days (P14) receiving an intravitreal injection of either scrambled miRNAs (control) or miR-143 mimics. Total RNAs were isolated from the retinas and subjected to RNA-Seq. Analysis of RNA-Seq data identified 2064 genes with altered levels between OIR rats and shame rats receiving scrambled RNAs (adjusted p<0.05).
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| Overall design |
Four groups of samples were analysed:
1) OIR with scrambled RNA (n=3)
2) OIR with miR-143 mimic (n=3)
3) Sham with scrambled RNA (n=3)
4) Sham with miR-143 mimic (n=3)
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| |
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| Contributor(s) |
Wang J, Singh V, Hewitt AW, Liu G |
| Citation(s) |
34797904 |
| Submission date |
Oct 04, 2017 |
| Last update date |
Nov 08, 2023 |
| Contact name |
vikrant singh |
| E-mail(s) |
vikrantvicky.1987@gmail.com
|
| Organization name |
Royal college of Surgeons in Ireland
|
| Street address |
31 York Street
|
| City |
Dublin |
| State/province |
Ireland |
| ZIP/Postal code |
D02YN77 |
| Country |
Ireland |
| |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA413139 |
| SRA |
SRP119398 |
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