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Series GSE104516 Query DataSets for GSE104516
Status Public on Sep 01, 2022
Title Gene expression data from sorted G1-G2/M phase of SLIMP knockdown and control cells.
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Drosophila melanogaster cells depleted of SLIMP showed a clear arrest in G2/M phase. The objective of this gene expression study was to determine the pathways involved in the phenotype of this cells.
 
Overall design Drosophila S2 stable cell lines were induced with CuSO4 to induce the knockdown of SLIMP. G1 and G2/M phases of control cells and SLIMP depleted cells were then sorted using a FacsAria Fusion sorter. Cell cycle from live cells was obtained with the incubation with Vybrant DyeCycle Violet Stain (Thermofisher). Two replicates for the SLIMP depleted cells and three replicates for the control cells were analysed.
 
Contributor(s) Antolin-Fontes A, Ribas de Pouplana L
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 02, 2017
Last update date Sep 03, 2022
Contact name Oscar Reina Garcia
E-mail(s) oscar.reina@irbbarcelona.org
Organization name IRB Barcelona
Department Biostatistics and Bioinformatics
Street address C/Baldiri Reixac 10
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (10)
GSM2802759 G1_control_rep1
GSM2802760 G2/M_control_rep1
GSM2802761 G1_SLIMPKD_rep2
Relations
BioProject PRJNA412890

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE104516_RAW.tar 20.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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