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| Status |
Public on Feb 01, 2008 |
| Title |
Gene expression data from Hexose-6-phosphate dehydrogenase knockout mouse muscle at 4 weeks |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Hexose-6-phosphate dehydrogenase (H6PD)is the initial component of a pentose phosphate pathway inside the endoplasmic reticulum (ER) that generates NADPH for ER enzymes. In liver, H6PD is required for the 11-oxoreductase activity of 11ss-hydroxysteroid dehydrogenase type 1 (11ss-HSD1), which converts inactive 11-oxo glucocorticoids to their active 11-hydroxyl counterparts; consequently, H6PD null mice are relatively insensitive to glucocorticoids, exhibiting fasting hypoglycemia, increased insulin sensitivity despite elevated circulating levels of corticosterone, and increased basal and insulin-stimulated glucose uptake in muscles normally enriched in Type II (fast) fibers which have increased glycogen content. They also display a progressive vacuolar myopathy evident after 4 weeks of age.
We carried out microarray analysis on TA and soleus muscles from 4 week old WT and KO mice to determine an expression profile predicting myopathy.
Keywords: Age dependant disease state phenotype comparison
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| Overall design |
4 week old mice are weaned and do not display overt histological evidence of myopathy. Soleus and tibialis anterior are used as comparison groups as they have distinct fibre type content and differing metabolic properties. 3 biological replicates are used for each genotpye and sample type.
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| Contributor(s) |
Lavery G, Turan N |
| Citation(s) |
18222920 |
| Submission date |
Jan 31, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Gareth Lavery |
| E-mail(s) |
g.g.lavery@bham.ac.uk
|
| Organization name |
University of Birmingham
|
| Street address |
2nd Floor IBR
|
| City |
Birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
|
| GSM261507 |
KO Soleus at 4 weeks biological rep1 |
| GSM261508 |
KO Soleus at 4 weeks biological rep2 |
| GSM261509 |
KO Soleus at 4 weeks biological rep3 |
| GSM261510 |
WT Soleus at 4 weeks biological rep1 |
| GSM261511 |
WT Soleus at 4 weeks biological rep2 |
| GSM261512 |
WT Soleus at 4 weeks biological rep3 |
| GSM261513 |
KO Tibialis Anterior at 4 weeks biological rep1 |
| GSM261514 |
KO Tibialis Anterior at 4 weeks biological rep2 |
| GSM261515 |
KO Tibialis Anterior at 4 weeks biological rep3 |
| GSM261516 |
WT Tibialis Anterior at 4 weeks biological rep1 |
| GSM261517 |
WT Tibialis Anterior at 4 weeks biological rep2 |
| GSM261518 |
WT Tibialis Anterior at 4 weeks biological rep3 |
|
| Relations |
| BioProject |
PRJNA105593 |
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