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| Status |
Public on Apr 26, 2008 |
| Title |
The Sf1-related nuclear hormone receptor Hr39 regulates Drosophila female reproductive tract development and function |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
The vertebrate nuclear hormone receptor steroidogenic factor 1 (SF1; NR5A1) controls reproductive development and regulates the transcription of steroid-modifying cytochrome P450 genes. We find that the SF1-related Drosophila nuclear hormone receptor HR39 is also essential for sexual development. In Hr39 mutant females, the sperm-storing spermathecae and glandular parovaria are absent or defective, causing sterility. Our results indicate that spermathecae and parovaria secrete reproductive tract proteins required for sperm maturation and function, like the mammalian epididymis and female reproductive tract. Hr39 controls the expression of specific cytochrome P450 genes and is required in females both to activate spermathecal secretion and repress male-specific courtship genes such as takeout. Thus, a pathway that, in vertebrates, controls sex-specific steroid hormone production, also mediates reproductive functions in an invertebrate. Our findings suggest that Drosophila can be used to model more aspects of mammalian reproductive biology than previously believed.
Keywords: mutant/wild-type comparison and tissue comparison
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| |
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| Overall design |
Wild type and Hr39(04443) Spermathecae, Wild type and Hr39(04443) Reproductive Tract
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| |
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| Contributor(s) |
Spradling AC, Allen AK |
| Citation(s) |
18077584 |
| Submission date |
Jan 24, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Allan C Spradling |
| E-mail(s) |
spradling@ciwemb.edu
|
| URL |
http://www.ciwemb.edu
|
| Organization name |
Carnegie Institution/HHMI
|
| Department |
Embryology
|
| Street address |
3520 San Martin Dr
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21218 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (8)
|
| GSM259196 |
Hr39(04443) Female Lower Reproductive Tract |
| GSM259197 |
Wild-type Female Lower Reproductive Tract |
| GSM259198 |
Hr39(04443) Female Spermathecae |
| GSM259199 |
Wild-type Female Spermathecae |
| GSM259201 |
Hr39(04443) Female Lower Reproductive Tract (duplicate) |
| GSM259202 |
Wild-type Female Lower Reproductive Tract (duplicate) |
| GSM259203 |
Hr39(04443) Female Spermathecae (duplicate) |
| GSM259204 |
Wild-type Female Spermathecae (duplicate) |
|
| Relations |
| BioProject |
PRJNA108461 |
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