|
| Status |
Public on Mar 01, 2018 |
| Title |
Gene expression in Sox9 progenitors in the developing mouse lung |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
This submission consists of two experiments utilizing translating ribosome affinity purification-sequencing (TRAP-seq). The first uses TRAP-seq to isolate transcripts from Sox9 positive progenitors at E14 and an E17 lung and compares it to non-immunoprecipitated whole lung homogenate control. The second compares TRAP-seq isolated transcripts from Sox9-wild type progenitors versus mutant progenitors. Recombination was induced at E13 and cells were isolated at E16.
|
| |
|
| Overall design |
Sox9-TRAP at E14 vs supernatant; Sox9-TRAP at E17 vs whole lung homogenate; duplicated Sox9-mutant versus Sox9-wild-type
|
| |
|
| Contributor(s) |
Chen J, Ostrin E |
| Citation(s) |
29440304 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL130129 |
Role of AT1 cells in perinatal lung maturation |
The University of Texas MD Anderson Cancer Center |
JICHAO CHEN |
|
| Submission date |
Jul 18, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Edwin Justin Ostrin |
| E-mail(s) |
ejostrin@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Pulmonary Medicine
|
| Street address |
1515 Holcombe Blvd, Unit 1465
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA394882 |
| SRA |
SRP112742 |
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