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| Status |
Public on Nov 26, 2019 |
| Title |
Whole transcriptome analysis of brain hippocampal tissue from SAMP8 mice and rat primary neurons treated with the Alzheimer's disease drug candidates CMS121 and J147 |
| Organisms |
Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Alzheimer's disease (AD) drug discovery has rarely been addressed in the context of aging even though sporadic AD accounts for 99% of the cases. Phenotypic screens based upon old age-associated brain toxicities were used to develop the potent AD drug candidates CMS121 and J147. The aim of this project was to investigate whether these two different AD drug candidates prevented the progression of dementia in SAMP8 mice when administered at advanced stages of disease, and whether they shared common modes of action. These transcriptomic data are part of an integrative multi-omics approach that also investigated protein expression, metabolite levels as well as cognition. In addition, in order to further investigate the effect of the drugs in in vitro neuronal cultures, rat primary neurons were treated with the compounds and the transcriptome sequenced.
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| Overall design |
Six nine-month old female SAMP8 mice per group were fed with vehicle diet and with CMS121 (400ppm) and J147 (200ppm) diets. Diet treatment lasted for four months until mice reached thirteen months of age. Mice were then sacrificed and RNA extracted from the brain hippocampus. Five eight-month old male SAMP8 mice were used as the baseline control group. For the in vitro experiments, Primary cortical neurons were prepared from day seventeen rat embryos and allowed to differentiate in culture. At seven days in vitro, neurons were treated with 1 M of each compound for 24h, and RNA purified
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| Contributor(s) |
Currais A, Huang L |
| Citation(s) |
31742554, 32884834, 33294943 |
| Submission date |
Jul 10, 2017 |
| Last update date |
Mar 28, 2022 |
| Contact name |
April Elizabeth Williams |
| E-mail(s) |
apriljack06@gmail.com, awilliams@salk.edu
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| Phone |
7345461645
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| Organization name |
Salk Institute for Biological Studies
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| Department |
IGC
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| Street address |
10010 N Torrey Pines Rd
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| City |
San Diego |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA393728 |
| SRA |
SRP111484 |
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