GEO help: Mouse over screen elements for information.
Status
Public on Jan 31, 2009
Title
MPIP24K
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Mus musculus Manufacturer
MPI of Psychiatry, Munich
Manufacture protocol
The cDNA microarray contains 24192 PCR products derived from different mouse cDNA libraries (sources: Research Genetics, RZPD Deutsches Ressourcenzentrum für Genomforschung, proprietary clones) representing 12387 different unigene clusters (unigene build #143). The spotting buffer contained 3 x SSC and 1.5 M betaine. The PCR products were spotted on GAPS II slides (Corning) using a Chipwriter Pro spotting robot (Biorad) and 48 SMP3 stealth pins (Telechem). After spotting, the arrays were heated to 80°C for 10 seconds and crosslinked using a UV Stratalinker 2400 at 200mJ (Stratagene). Slides were blocked 30 min at 65°C and prehybridized for 1 h at 42°C in mixture with 0.1 mg/ml BSA, 50% formamide, 5 x SSC and 0.1 x SDS. The arrays were rinsed with ultrapure water, then vigourously shaken for a few seconds in a 50 ml polypropylene tube containing isopropanol. The arrays were dried by centrifugation for 2 minutes at 400 g and hybridized within a few days.
Submission date
Oct 10, 2008
Last update date
Jan 31, 2009
Contact name
Dietrich Trümbach
E-mail(s)
dietrich.truembach@helmholtz-muenchen.de Organization name
German Research Center for Environmental Health
Department
Institute for Developmental Genetics
Street address
Ingolstädter Landstraße 1
City
München-Neuherberg
ZIP/Postal code
85764
Country
Germany
Samples (160)
GSM329341 , GSM329342 , GSM329343 , GSM329344 , GSM329345 , GSM329346
GSM329347 ,
GSM329348 ,
GSM329349 ,
GSM329350 ,
GSM329351 ,
GSM329352 ,
GSM329353 ,
GSM329354 ,
GSM329355 ,
GSM329356 ,
GSM329357 ,
GSM329358 ,
GSM329359 ,
GSM329360 ,
GSM329361 ,
GSM329362 ,
GSM329363 ,
GSM329364 ,
GSM329365 ,
GSM329366 ,
GSM329367 ,
GSM329368 ,
GSM329369 ,
GSM329370 ,
GSM366775 ,
GSM366776 ,
GSM366777 ,
GSM366778 ,
GSM366779 ,
GSM366780 ,
GSM366781 ,
GSM366782 ,
GSM366783 ,
GSM366784 ,
GSM521999 ,
GSM522000 ,
GSM522001 ,
GSM522002 ,
GSM522003 ,
GSM522004 ,
GSM522005 ,
GSM522006 ,
GSM522007 ,
GSM522008 ,
GSM522009 ,
GSM522010 ,
GSM522011 ,
GSM522012 ,
GSM522013 ,
GSM522014 ,
GSM522015 ,
GSM522016 ,
GSM522017 ,
GSM522018 ,
GSM522019 ,
GSM522020 ,
GSM522021 ,
GSM522022 ,
GSM522023 ,
GSM522024 ,
GSM522025 ,
GSM522026 ,
GSM522027 ,
GSM522028 ,
GSM522029 ,
GSM522030 ,
GSM522031 ,
GSM522032 ,
GSM522033 ,
GSM522034 ,
GSM522035 ,
GSM522036 ,
GSM522037 ,
GSM522038 ,
GSM522039 ,
GSM522040 ,
GSM522041 ,
GSM522042 ,
GSM522043 ,
GSM522044 ,
GSM522045 ,
GSM522046 ,
GSM522047 ,
GSM522048 ,
GSM718523 ,
GSM718524 ,
GSM718525 ,
GSM718526 ,
GSM718527 ,
GSM718528 ,
GSM718529 ,
GSM718530 ,
GSM718531 ,
GSM718532 ,
GSM718533 ,
GSM718534 ,
GSM718535 ,
GSM718536 ,
GSM718537 ,
GSM718538 ,
GSM718539 ,
GSM718540 ,
GSM718541 ,
GSM718542 ,
GSM718543 ,
GSM718544 ,
GSM718545 ,
GSM718546 ,
GSM718547 ,
GSM718548 ,
GSM718549 ,
GSM718550 ,
GSM718551 ,
GSM718552 ,
GSM718553 ,
GSM718554 ,
GSM718555 ,
GSM718556 ,
GSM718557 ,
GSM718558 ,
GSM718559 ,
GSM718560 ,
GSM718561 ,
GSM718562 ,
GSM718563 ,
GSM718564 ,
GSM718565 ,
GSM718566 ,
GSM718567 ,
GSM718568 ,
GSM718569 ,
GSM718570 ,
GSM718571 ,
GSM718572 ,
GSM718573 ,
GSM718574 ,
GSM718575 ,
GSM718576 ,
GSM718577 ,
GSM718578 ,
GSM718579 ,
GSM718580 ,
GSM718581 ,
GSM718582 ,
GSM718583 ,
GSM718584 ,
GSM718585 ,
GSM718586 ,
GSM718587 ,
GSM718588 ,
GSM718589 ,
GSM718590 ,
GSM718591 ,
GSM718592 Series (5)
GSE13156 Murine corticotroph pituitary AtT-20 cells: Crh treatment time course
GSE14687 Involvement of the brain renin-angiotensin system in the effects of maternal separation
GSE20877 Novel aspects of the transcriptional stress response in the paraventricular nucleus of the mouse strains DBA/2J and C57BL/6J
GSE28999 Gene expression profiling of high (HAB) vs. low (LAB) anxiety-related behavior mice in seven brain regions.
GSE29015 Gene expression analyses of mice selectively inbred for trait anxiety
Data table header descriptions ID Clone number of sequence used to define oligonucleotide probe
GB_ACC NCBI accession number of sequence used to design oligonucleotide probe GenBank Accession number
gene_symbol Gene symbols
gene_name Gene description
GENE Entrez Gene ID
REFSEQ NCBI Reference Sequences ID
GI NCBI gi identifier
prot_acc Refseq protein identifier
SPOT_ID
Data table ID GB_ACC gene_symbol gene_name GENE REFSEQ GI prot_acc SPOT_ID 3
AI447914 12
AI852469 17
AA386815 20
-- NO INFORMATION
21
-- NO INFORMATION
41
-- NO INFORMATION
42
-- NO INFORMATION
47
AI835568 59
AA289059 62
-- NO INFORMATION
63
-- NO INFORMATION
84
-- NO INFORMATION
104
-- NO INFORMATION
105
-- NO INFORMATION
126
-- NO INFORMATION
134
AI846638 135
AI842116 146
-- NO INFORMATION
147
-- NO INFORMATION
149
AI428008
24192 2057 Kbytes .Supplementary data files not provided
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