Slide spotted using a MicroGrid II (Genomic Solution) Spotting conditions: Temperature of 20¡C more or less 5¡C. Hygroscopy of 50% more or less 5%. Before each spotting: 1. Printing tips (Geneworx 10k) are tested on glass slides. 2. The spotting robot is put in operation and water tanks filled with distilled water. 3. Corning glass slides are deposited on plates. 4. The spotting program specific for each slide bacth is launched.
Support
Glass slide
Coating
Amino silane (Corning UltraGAPS)
Description
This cDNA clone set was prepared by researchers at the National Institute on Aging. The clone set has been distributed widely and is currently in use by a number of other microarray programmes. The set has the following characteristics: ~ 15,000 'unique' cDNA clones were rearrayed from 52,374 ESTs from pre- and periimplantation embryos, E12.5 female gonad/mesonephros, and newborn ovary. Up to 50% are derived from novel genes. ~ 1.5 kb average insert size. Clones were sequenced from 5' and 3' termini to obtain longer reads and verify sequence. Sequence information is available from their web site (NIA/NIH Mouse Genomics Home Page). PCR products were amplified by the National Sequencing Center.
Identification of Phox2b-regulated genes by expression profiling of cranial motoneuron precursors
Data table header descriptions
ID
NIA clonest identifier
Parental_Clone
Source clone name
Clone_Verification
Either the 3' sequence or the 5' sequence show sufficient homology to verify the address of the clone is correct
Full_Seq
Value=Y indicates full sequence for the clone is available. 3' sequence and 5' sequence assembled using Phred/Phrap system
Sequence_name_3'-end
3' sequence of the Clone (daughter clone)
3'-sequence_verified
The 3' sequence of the Daughter clone shows sufficient homology to the 3' parent clone to verify the sequence. Values are Y= verified, U= Unvarifiable, <blank>=not verified, O=Orphan
Length
Length of 3' sequence of the Clone (daughter clone)
GB_ACC_3
GenBank Accession number of the 3' end Sequence of the Clone (daughter clone)
3'_Putative_Gene_ID
Putative ID based on BLAST search of the 3' sequence
3'_Putative_Gene_Symbol
Putative Gene symbol based on BLAST search of the 3' sequence
NIA_cluster_3'
NIA Mouse Gene Index version 4 cluster in which 3' Sequence present
Sequence_name_5'-end
5' sequence of the Clone (daughter clone)
5'-sequence_verified
The 3' sequence of the Daughter clone shows sufficient homology to the 3' parent clone to verify the sequence. Values are Y= verified, U= Unvarifiable, <blank>=not verified, O=Orphan
Length
GB_ACC_5
GenBank Accession number of the 5' end Sequence of the Clone (daughter clone)
5'_Putative_Gene_ID
Putative ID based on BLAST search of the 5' sequence
5'_Putative_Gene_Symbol
Putative Gene symbol based on BLAST search of the 5' sequence
NIA_cluster_5'
NIA Mouse Gene Index version 4 cluster in which 5' Sequence present
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