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Platform GPL5458 Query DataSets for GPL5458
Status Public on Jul 11, 2007
Title HGMP NIA 15 K, slide 21-40 (2 of 2). Printed by HGMP, Hinxton
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Mus musculus
Manufacturer HGMP, Hinxton UK
Manufacture protocol cDNA arrays (now discontinued), spotted PCR product. Bacterial source material diluted 1/10 prior to PCR,
primers: Ko forward primer (amidated): GTG TGG AAT TGT GAG CGG ATA ACA A (25mer) with 5' NH2(C6) modification.
Ko reverse primer: CCA GTC ACG ACG TTG TAA AAC GAC (24mer). Purified using Millipore Multiscreen PCR plates. Purified PCR products have then been rearrayed into 384 well plates and printed. 20µl of spotting buffer is added to 10µl of PCR product (0.1ug/ul) and this is sufficient to grid over 1000 glass arrays.
The manufacturers ask that the following paper describing the clones is cited in connection with these arrays: Tanaka TS, et al Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. PNAS (2000) 97: 9127-32
Catalog number non- commercial
Support glass
Coating aminosilane
 
Description Second slide of 2. HGMP printed NIA 15K set, over 2 slides, 17280 spots per slide, including empties, control spots, landmark spots, and duplicate experimental spots. 12 rows and 4 columns of grids, each grid contains 18 rows and 20 columns of spots (format for both slides 1-20 and 21-40).
 
Contributor(s) Mansergh FC
Citation(s) 10922068
Submission date Jul 02, 2007
Last update date May 29, 2008
Contact name Fiona Catherine Mansergh
E-mail(s) mansergf@tcd.ie
Phone 0035318962484
Organization name Cardiff University
Department Biosciences
Lab Prof Sir Martin Evans
Street address Museum Avenue
City Cardiff
State/province Wales
ZIP/Postal code CF10 3US
Country United Kingdom
 
Samples (10) GSM207397, GSM207401, GSM207406, GSM207414, GSM207471, GSM208596 
Series (1)
GSE8381 SparcTm1Cam129SvEv bone vs 129Sv/Evcontrol bone

Data table header descriptions
ID
Address describes exact position of spot on the array
meta_row 12 rows of grids on the slide, describes in which the spot is located
meta_col 4 columns of grids on the slide, describes in which the spot is located
row1 describes spot location in one of 18 rows in a grid
col describes spot location in one of 20 columns in a grid
GB# GenBank accession number (or empty spot, control spot or landmark)
GB_ACC GenBank accession number
SPOT_ID spot identifier

Data table
ID Address meta_row meta_col row1 col GB# GB_ACC SPOT_ID
HG NIA 1 a1.a1 1 1 1 1 :landing light 1 --CONTROL_landing light 1
HG NIA 2 a1.a10 1 1 1 10 >empty 1 --empty 1
HG NIA 3 a1.a11 1 1 1 11 >empty 2 --empty 2
HG NIA 4 a1.a12 1 1 1 12 >empty 3 --empty 3
HG NIA 5 a1.a13 1 1 1 13 #U47810 --CONTROL
HG NIA 6 a1.a14 1 1 1 14 #U49720 --CONTROL
HG NIA 7 a1.a15 1 1 1 15 #X04752 --CONTROL
HG NIA 8 a1.a16 1 1 1 16 #X57497 --CONTROL
HG NIA 9 a1.a17 1 1 1 17 #X61600 --CONTROL
HG NIA 10 a1.a18 1 1 1 18 #X74342 --CONTROL
HG NIA 11 a1.a19 1 1 1 19 #Z46663 --CONTROL
HG NIA 12 a1.a2 1 1 1 2 #M27938 --CONTROL
HG NIA 13 a1.a20 1 1 1 20 :landing light 2 --CONTROL_landing light 2
HG NIA 14 a1.a3 1 1 1 3 #D45022 --CONTROL
HG NIA 15 a1.a4 1 1 1 4 #M26926 --CONTROL
HG NIA 16 a1.a5 1 1 1 5 #M36780 --CONTROL
HG NIA 17 a1.a6 1 1 1 6 #M61737 --CONTROL
HG NIA 18 a1.a7 1 1 1 7 #M87863 --CONTROL
HG NIA 19 a1.a8 1 1 1 8 #M98454 --CONTROL
HG NIA 20 a1.a9 1 1 1 9 >empty 4 --empty 4

Total number of rows: 17280

Table truncated, full table size 805 Kbytes.






Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided
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