GEO help: Mouse over screen elements for information.
Status
Public on Feb 28, 2015
Title
Custom PEPperCHIP Peptide Microarray
Technology type
spotted peptide or protein
Distribution
custom-commercial
Organism
Homo sapiens Manufacturer
PEPperPRINT
Manufacture protocol
Amino acid microparticles are laser printed onto a glass slide with a custom 24-color laser printer. One layer of an amino acid particle pattern is printed on a coated glass slide. A heating step melts the particles. Embedded amino acids can couple to the chip surface. In accordance with solid phase Fmoc chemistry, a cycle of synthesis is completed after removal of excessive material by washing and cleavage of the N-terminal Fmoc protection group. The cycle is repeated until the full peptide microarray is synthesized.
Description
Laser printer spotted peptides (peptide autoantigens).
Submission date
Jan 23, 2015
Last update date
Feb 28, 2015
Contact name
Jennie Hamilton
Organization name
University of Alabama Birmingham
Department
Department of Medicine, Division of Clinical Immunology and Rheumatology
Street address
1825 University Blvd.
City
Birmingham
State/province
AL
ZIP/Postal code
35294
Country
USA
Samples (24)
GSM1590409 , GSM1590410 , GSM1590411 , GSM1590412 , GSM1590413 , GSM1590414
GSM1590415 ,
GSM1590416 ,
GSM1590417 ,
GSM1590418 ,
GSM1590419 ,
GSM1590420 ,
GSM1590421 ,
GSM1590422 ,
GSM1590423 ,
GSM1590424 ,
GSM1590425 ,
GSM1590426 ,
GSM1590427 ,
GSM1590428 ,
GSM1590429 ,
GSM1590430 ,
GSM1590431 ,
GSM1590432 Series (2)
GSE65234 Autoimmune peptide antigen IgG and IgM reactivity in B6 and BXD2 mice with spontaneous systemic autoimmune disease
GSE65290 General Approach for Tetramer Based Identification of Autoantigen Reactive B Cells: Characterization of La and snRNP Reactive B Cells in Autoimmune BXD2 Mice
Data table header descriptions ID Antigen Source Molecule Peptide Source Antigen
SEQUENCE Peptide amino acid sequence
Data table ID Antigen Source Molecule SEQUENCE GAEGHWRRLP
2-Oxoisovalerate dehydrogenase subunit beta (mitochondrial)
GAEGHWRRLP
AEFVQQKNFLVEEEQRQ
52 kDa Ro protein
AEFVQQKNFLVEEEQRQ
EVTCPICLDPFVEPV
52 kDa Ro protein
EVTCPICLDPFVEPV
LAQQSQALQELISELDR
52 kDa Ro protein
LAQQSQALQELISELDR
LQELEKDEREQ
52 kDa Ro protein
LQELEKDEREQ
EGMRFDKGYISGYFV
60 kDa chaperonin 2
EGMRFDKGYISGYFV
LLRVIPALDSLTPANED
60 kDa heat shock protein
LLRVIPALDSLTPANED
ALSSQHQARIEIESFYE
78 kDa glucose-regulated protein
ALSSQHQARIEIESFYE
TITNDQNRLTPEEIERM
78 kDa glucose-regulated protein
TITNDQNRLTPEEIERM
ALSSQHQAZIEIESFYE
78 kDa glucose-regulated protein [Cit]
ALSSQHQAZIEIESFYE
TITNDQNZLTPEEIERM
78 kDa glucose-regulated protein also Bip [Cit]
TITNDQNZLTPEEIERM
GDTHLGGEDFDQRVMEH
78 kDa GRP (BiP)
GDTHLGGEDFDQRVMEH
QNRLTPEEIERMVNDAE
78 kDa GRP (BiP)
QNRLTPEEIERMVNDAE
SRKPEAPRRR
centromere protein-A limited
SRKPEAPRRR
PDREFLLRVSYMEIY
Centromere-associated protein E
PDREFLLRVSYMEIY
DPRYTCQREF
Citrate synthase
DPRYTCQREF
NWVSRLANLTQGEDQYY
Clusterin
NWVSRLANLTQGEDQYY
NWVSZLANLTQGEDQYY
Clusterin [Cit]
NWVSZLANLTQGEDQYY
GARGLTGRPGDA
Collagen alpha-1(II) chain
GARGLTGRPGDA
GAZGLTGZPGDA
Collagen alpha-1(II) chain [cit] (R3, R8)
GAZGLTGZPGDA
86 5 Kbytes .Supplementary data files not provided
Less...
More...