GEO help: Mouse over screen elements for information.
Status
Public on Nov 15, 2014
Title
Broad P. falciparum 328 v1
Technology type
other
Distribution
custom-commercial
Organism
Plasmodium falciparum Manufacturer
Nanostring Technologies
Manufacture protocol
From Methods section of associated publication: nCounter® probe specificity is generated by designing two 50bp probes that sit next to one another, yielding 100bp total probe specificity for each gene of interest. Probe set design was carried out to maximize specificity and minimize cross-hybridization. Specifically, long direct and inverted repeats and long polynucleotide repeats were excluded, target regions were screened for cross-hybridization against the Human RefSeq mRNA database (http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/refseq/ ), and probes with stretches of more than 15 contiguous bases complementary to any non-target mRNA were excluded. Probes were also screened for inter- and intra-probe interactions, and were selected to have calculated melting temperatures (Tm) between 78 and 83°C, with an ideal target of 80.5°C. Finally, mismatch probes were also included in the nCounter® custom probe set, as part of the negative controls.
Submission date
Nov 13, 2014
Last update date
Nov 15, 2014
Contact name
Daria Van Tyne
Organization name
Harvard Medical School
Street address
243 Charles Street
City
Boston
ZIP/Postal code
02114
Country
USA
Samples (89)
GSM1544559 , GSM1544560 , GSM1544561 , GSM1544562 , GSM1544563 , GSM1544564
GSM1544565 ,
GSM1544566 ,
GSM1544567 ,
GSM1544568 ,
GSM1544569 ,
GSM1544570 ,
GSM1544571 ,
GSM1544572 ,
GSM1544573 ,
GSM1544574 ,
GSM1544575 ,
GSM1544576 ,
GSM1544577 ,
GSM1544578 ,
GSM1544579 ,
GSM1544580 ,
GSM1544581 ,
GSM1544582 ,
GSM1544583 ,
GSM1544584 ,
GSM1544585 ,
GSM1544586 ,
GSM1544587 ,
GSM1544588 ,
GSM1544589 ,
GSM1544590 ,
GSM1544591 ,
GSM1544592 ,
GSM1544593 ,
GSM1544594 ,
GSM1544595 ,
GSM1544596 ,
GSM1544597 ,
GSM1544598 ,
GSM1544599 ,
GSM1544600 ,
GSM1544601 ,
GSM1544602 ,
GSM1544603 ,
GSM1544604 ,
GSM1544605 ,
GSM1544606 ,
GSM1544607 ,
GSM1544608 ,
GSM1544609 ,
GSM1544610 ,
GSM1544612 ,
GSM1544614 ,
GSM1544615 ,
GSM1544617 ,
GSM1544619 ,
GSM1544620 ,
GSM1544622 ,
GSM1544624 ,
GSM1544625 ,
GSM1544626 ,
GSM1544627 ,
GSM1544628 ,
GSM1544629 ,
GSM1544630 ,
GSM1544631 ,
GSM1544632 ,
GSM1544633 ,
GSM1544634 ,
GSM1544635 ,
GSM1544636 ,
GSM1544637 ,
GSM1544638 ,
GSM1544639 ,
GSM1544640 ,
GSM1544641 ,
GSM1544642 ,
GSM1544643 ,
GSM1544644 ,
GSM1544645 ,
GSM1544646 ,
GSM1544647 ,
GSM1544648 ,
GSM1544649 ,
GSM1544650 ,
GSM1544651 ,
GSM1544652 ,
GSM1544653 Series (1)
GSE63260 Plasmodium falciparum gene expression measured directly from tissue during human infection
Data table header descriptions ID Code Class Positive and negative control probes, Endogenous probes are P. falciparum genes
Probe/Gene Name Name of probe, name of P. falciparum gene
ORF ORF Name
SEQUENCE Probe sequences for P. falciparum genes
SPOT_ID
Data table ID Code Class Probe/Gene Name ORF SEQUENCE SPOT_ID POS_A(128)
Positive
POS_A(128)
Proprietary
POS_B(32)
Positive
POS_B(32)
Proprietary
POS_C(8)
Positive
POS_C(8)
Proprietary
POS_D(2)
Positive
POS_D(2)
Proprietary
POS_E(0.5)
Positive
POS_E(0.5)
Proprietary
POS_F(0.125)
Positive
POS_F(0.125)
Proprietary
NEG_A(0)
Negative
NEG_A(0)
Proprietary
NEG_B(0)
Negative
NEG_B(0)
Proprietary
NEG_C(0)
Negative
NEG_C(0)
Proprietary
NEG_D(0)
Negative
NEG_D(0)
Proprietary
NEG_E(0)
Negative
NEG_E(0)
Proprietary
NEG_F(0)
Negative
NEG_F(0)
Proprietary
NEG_G(0)
Negative
NEG_G(0)
Proprietary
NEG_H(0)
Negative
NEG_H(0)
Proprietary
MAL13P1.122
Endogenous
MAL13P1.122
MAL13P1.122 AAAAAACGGAAAAGACTGGGTATGGTGTGTTTTGCAAGAGGGACATAAAAAATGGAGAACTCATATGTGAATACGTTGGAGAAGTATTAGGAAAACGCGA
MAL13P1.123
Endogenous
MAL13P1.123
MAL13P1.123 GACAAGTATGTGCTTTCTCATTTAGAAGGAATATCTGAGAACATCAGGCATATGGTAAAATTAAAAGAGGACGTAGAGAAGTTTGTAAGTAAGCACGGGT
MAL13P1.130
Endogenous
MAL13P1.130
MAL13P1.130 CCATGGGAACTGTATCATGGAGCTTGTCCCTTATATGTTTGATGGCCTCTTCGTACTATTTTGATGACCCTTGGATGGAAGAAAAGATAGGTGCAGGTTC
MAL13P1.184
Endogenous
MAL13P1.184
MAL13P1.184 AAATCCCTCTAAACGATTTCTTGGATTATTATCAGGAAAATAAAGAGGACAAATACACATCAAGGAGCTCTCAATTTAACCAGATAATGAGGTTCTTAAT
MAL13P1.190
Endogenous
MAL13P1.190
MAL13P1.190 GATCAACGATATTGGCAAAAAGATTGGCATTGAGTCGTCCCTCGATGTGGTGGGAATAACTGCAAAAGCCATTCACGACGGTGTGATTGAAGGAACTATT
MAL13P1.195
Endogenous
MAL13P1.195
MAL13P1.195 AATCTATTTATATGATGAGCAAAAGGCAAGCTGGGCAGAAATATGCGAAGTAAACTGGCCGTGTATGCCCTTGGAGTGTTCATATGGTAGAGACTACAAT
342 46 Kbytes .Supplementary data files not provided
Less...
More...