The Operon version 1.1 oligonucleotide probe set (70-mers) was printed in the Massachusetts General Hospital microarray core facility using an OmniGrid 100 (GeneMachines) on CodeLink activated slides (Amersham). Oligos were printed in 150 mM sodiumphosphate pH 8.5 with 0.0005% N-lauroyl sarcosine. Oligos were coupled to the activated slides by allowing the aminolinker on the oligos to react over night in a humidity chamber. Unreacted groups on the slide surface were blocked with 150 mM glycine, 0.1 M tris, 0.1% N-lauroyl sarcosine, pH 9. Keywords = spotted, long oligonucleotide, Operon
LPS-induced and E. coli-induced gene expression in wildtype and MyD88-/- mouse macrophages
Data table header descriptions
ID
GB_ACC
GenBank accession number of sequence used to design oligonucleotide probe;
OLIGO_ID
Oligonucleotide probe ID number
CONTROL
A 'C' in this column indicates a control spot of some kind - 'BLANK', buffer only; 'EMPTY', not printed; 'positive control', a mixture of all oligos printed; 'negative control', oligonucleotide designed to not hybridize to mouse genome
TIP_ID
Print tip ID
ROW
Row within a subgrid printed by a print tip
COLUMN
Column within a subgrid printed by a print tip
SPOT_ID
Column added by GEO staff to facilitate sequence tracking in Entrez GEO
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