Med25 mediator complex subunit 25 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Med25provided by MGI
Official Full Name: mediator complex subunit 25provided by MGI
Primary source: MGI:MGI:1922863
See related: Ensembl:ENSMUSG00000002968 AllianceGenome:MGI:1922863
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ESTM2; 2610034E13Rik; 2610529E18Rik
Summary: Enables retinoid X receptor binding activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Part of transcription regulator complex. Is expressed in central nervous system; incisor; submandibular gland primordium; and tail dorsal root ganglion. Orthologous to human MED25 (mediator complex subunit 25). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in adrenal adult (RPKM 84.2), ovary adult (RPKM 55.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 B3; 7 28.95 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (44526189..44544771, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (44876765..44892705, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Med25 Limits Master Regulators That Govern Adipogenesis.
Title: Med25 Limits Master Regulators That Govern Adipogenesis.
the identification of MED25 as one of the HNF4alpha binding partners in pancreatic ss-cells leading to insulin secretion which is impaired in MODY patients
Title: MED25 is a mediator component of HNF4α-driven transcription leading to insulin secretion in pancreatic beta-cells.
Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease.
Title: Identification of the variant Ala335Val of MED25 as responsible for CMT2B2: molecular data, functional studies of the SH3 recognition motif and correlation between wild-type MED25 and PMP22 RNA levels in CMT1A animal models.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2)
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

NoName (e-PCR)
- UniSTS:235660

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables nuclear retinoic acid receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables nuclear retinoic acid receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear retinoid X receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables nuclear retinoid X receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in RNA polymerase II preinitiation complex assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of fibroblast proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chromatin binding	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mediator complex assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription elongation by RNA polymerase II	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of core mediator complex	ISO Inferred from Sequence Orthology more info
part_of mediator complex	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription regulator complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: mediator of RNA polymerase II transcription subunit 25

Names: mediator of RNA polymerase II transcription, subunit 9 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001331206.2 → NP_001318135.1 mediator of RNA polymerase II transcription subunit 25 isoform 2

Status: VALIDATED

Source sequence(s)

AC158231

UniProtKB/TrEMBL

A0A140LHQ0
NM_001331207.2 → NP_001318136.1 mediator of RNA polymerase II transcription subunit 25 isoform 3

Status: VALIDATED

Source sequence(s)

AC158231

Consensus CDS

CCDS90228.1

UniProtKB/TrEMBL

A0A140LHG7, A0A140LHQ0

Related

ENSMUSP00000146405.2, ENSMUST00000208253.2
NM_001331208.2 → NP_001318137.1 mediator of RNA polymerase II transcription subunit 25 isoform 4

Status: VALIDATED

Source sequence(s)

AC158231

Consensus CDS

CCDS85291.1

UniProtKB/Swiss-Prot

Q8VCB2

Related

ENSMUSP00000146595.2, ENSMUST00000207278.2

Conserved Domains (5) summary

pfam09770
Location:459 → 586

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:297 → 442

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 280

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:530 → 618

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
NM_001415795.1 → NP_001402724.1 mediator of RNA polymerase II transcription subunit 25 isoform 5

Status: VALIDATED

Source sequence(s)

AC158231
NM_029365.4 → NP_083641.1 mediator of RNA polymerase II transcription subunit 25 isoform 1

See identical proteins and their annotated locations for NP_083641.1

Status: VALIDATED

Source sequence(s)

AC158231

Consensus CDS

CCDS21220.1

UniProtKB/Swiss-Prot

Q3UDX6, Q3UFQ1, Q6IR27, Q8VCB2

UniProtKB/TrEMBL

A0A140LHQ0

Related

ENSMUSP00000003049.7, ENSMUST00000003049.8

Conserved Domains (4) summary

pfam11232
Location:400 → 545

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:228 → 383

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:663 → 745

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:13 → 225

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

44526189..44544771 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017312366.3 → XP_017167855.1 mediator of RNA polymerase II transcription subunit 25 isoform X1

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:620 → 779

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:460 → 605

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:287 → 443

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:717 → 773

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:74 → 285

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
XM_030243066.2 → XP_030098926.1 mediator of RNA polymerase II transcription subunit 25 isoform X2

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:618 → 750

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:459 → 604

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:287 → 442

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:692 → 748

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:74 → 285

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
XM_036153534.1 → XP_036009427.1 mediator of RNA polymerase II transcription subunit 25 isoform X7

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (6) summary

pfam09770
Location:483 → 614

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:297 → 442

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 280

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:554 → 610

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam18658
Location:625 → 683

zf-C2H2_12; Zinc-finger C2H2-type

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_030243068.2 → XP_030098928.1 mediator of RNA polymerase II transcription subunit 25 isoform X8

Conserved Domains (5) summary

pfam09770
Location:460 → 589

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:298 → 443

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 281

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:531 → 587

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_006541265.4 → XP_006541328.1 mediator of RNA polymerase II transcription subunit 25 isoform X6

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:458 → 617

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:298 → 443

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 281

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:555 → 611

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_030243067.2 → XP_030098927.1 mediator of RNA polymerase II transcription subunit 25 isoform X7

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (6) summary

pfam09770
Location:483 → 614

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:297 → 442

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 280

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:554 → 610

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam18658
Location:625 → 683

zf-C2H2_12; Zinc-finger C2H2-type

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_017312368.2 → XP_017167857.1 mediator of RNA polymerase II transcription subunit 25 isoform X6

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:458 → 617

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:298 → 443

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 281

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:555 → 611

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_006541264.3 → XP_006541327.1 mediator of RNA polymerase II transcription subunit 25 isoform X5

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:621 → 748

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:459 → 604

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:287 → 442

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:692 → 780

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:74 → 285

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
XM_017312367.3 → XP_017167856.1 mediator of RNA polymerase II transcription subunit 25 isoform X4

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:622 → 749

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:460 → 605

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:287 → 443

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:693 → 781

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

pfam11265
Location:74 → 285

Med25_VWA; Mediator complex subunit 25 von Willebrand factor type A
XM_030243073.2 → XP_030098933.1 mediator of RNA polymerase II transcription subunit 25 isoform X12

Conserved Domains (5) summary

pfam09770
Location:459 → 586

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:297 → 442

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 280

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:530 → 618

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_030243070.2 → XP_030098930.1 mediator of RNA polymerase II transcription subunit 25 isoform X10

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:457 → 595

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:297 → 442

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 280

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:554 → 642

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_030243071.2 → XP_030098931.1 mediator of RNA polymerase II transcription subunit 25 isoform X10

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:457 → 595

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:297 → 442

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 280

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:554 → 642

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_030243072.2 → XP_030098932.1 mediator of RNA polymerase II transcription subunit 25 isoform X11

Conserved Domains (5) summary

pfam09770
Location:460 → 587

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:298 → 443

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 281

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:531 → 619

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...
XM_030243069.2 → XP_030098929.1 mediator of RNA polymerase II transcription subunit 25 isoform X9

UniProtKB/TrEMBL

A0A140LHQ0

Conserved Domains (5) summary

pfam09770
Location:458 → 596

PAT1; Topoisomerase II-associated protein PAT1

pfam11232
Location:298 → 443

Med25; Mediator complex subunit 25 PTOV activation and synapsin 2

pfam11235
Location:125 → 281

Med25_SD1; Mediator complex subunit 25 synapsin 1

pfam11244
Location:555 → 643

Med25_NR-box; Mediator complex subunit 25 C-terminal NR box-containing

cl00057
Location:1 → 123

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...