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    Themis thymocyte selection associated [ Mus musculus (house mouse) ]

    Gene ID: 210757, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Themisprovided by MGI
    Official Full Name
    thymocyte selection associatedprovided by MGI
    Primary source
    MGI:MGI:2443552
    See related
    Ensembl:ENSMUSG00000049109 AllianceGenome:MGI:2443552
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Gasp; Spot; Tsepa; thylex; E430004N04Rik
    Summary
    This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
    Expression
    Restricted expression toward thymus adult (RPKM 8.8) See more
    Orthologs
    human all
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    Genomic context

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    Location:
    10 A4; 10 16.06 cM
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (28544356..28759816)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (28668360..28883820)

    Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_26159 Neighboring gene slowmo homolog 2 pseudogene Neighboring gene protein tyrosine phosphatase receptor type K Neighboring gene STARR-seq mESC enhancer starr_26161 Neighboring gene STARR-seq mESC enhancer starr_26163 Neighboring gene STARR-seq mESC enhancer starr_26164 Neighboring gene STARR-seq mESC enhancer starr_26167 Neighboring gene STARR-seq mESC enhancer starr_26168 Neighboring gene CapStarr-seq enhancer MGSCv37_chr10:28384986-28385169 Neighboring gene STARR-seq mESC enhancer starr_26169 Neighboring gene STARR-seq mESC enhancer starr_26170 Neighboring gene pyruvate dehydrogenase E1 alpha 1 pseudogene Neighboring gene RIKEN cDNA 2310057J18 gene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Themis suppresses the effector function of CD8(+) T cells in acute viral infection. Tang J, et al. Cell Mol Immunol, 2023 May. PMID 36977779,
    2. Themis is indispensable for IL-2 and IL-15 signaling in T cells. Liu Y, et al. Sci Signal, 2022 Feb 15. PMID 35167340
    3. Expansion of an Unusual Virtual Memory CD8(+) Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice. Prasad M, et al. Front Immunol, 2021. PMID 33897691, Free PMC Article
    4. Proteomic Analysis of Regulatory T Cells Reveals the Importance of Themis1 in the Control of Their Suppressive Function. Duguet F, et al. Mol Cell Proteomics, 2017 Aug. PMID 28373295, Free PMC Article
    5. In vivo functional mapping of the conserved protein domains within murine Themis1. Zvezdova E, et al. Immunol Cell Biol, 2014 Sep. PMID 24935457, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Positive regulation of Vav1 by Themis controls CD4 T cell pathogenicity in a mouse model of central nervous system inflammation.
      Title: Positive regulation of Vav1 by Themis controls CD4 T cell pathogenicity in a mouse model of central nervous system inflammation.
    2. Themis controls T cell activation, effector functions, and metabolism of peripheral CD8[+] T cells.
      Title: Themis controls T cell activation, effector functions, and metabolism of peripheral CD8(+) T cells.
    3. Themis suppresses the effector function of CD8[+] T cells in acute viral infection.
      Title: Themis suppresses the effector function of CD8(+) T cells in acute viral infection.
    4. Themis is indispensable for IL-2 and IL-15 signaling in T cells.
      Title: Themis is indispensable for IL-2 and IL-15 signaling in T cells.
    5. Expansion of an Unusual Virtual Memory CD8(+) Subpopulation Bearing Valpha3.2 TCR in Themis-Deficient Mice.
      Title: Expansion of an Unusual Virtual Memory CD8(+) Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.
    6. THEMIS-SHP1 Recruitment by 4-1BB Tunes LCK-Mediated Priming of Chimeric Antigen Receptor-Redirected T Cells
      Title: THEMIS-SHP1 Recruitment by 4-1BB Tunes LCK-Mediated Priming of Chimeric Antigen Receptor-Redirected T Cells.
    7. Our analysis of post-selection Themis deletion identifies a novel function of Themis in mediating responses to subactivation threshold avidities of pMHC ligands and cytokines in peripheral CD8+ T cells
      Title: T cell receptor and cytokine signal integration in CD8(+) T cells is mediated by the protein Themis.
    8. This positive regulation of Shp1 activity by Themis is found in thymocytes, but not in peripheral T cells.
      Title: Themis-associated phosphatase activity controls signaling in T cell development.
    9. this study demonstrates the potential role of Themis1 in the suppressive functions of regulatory T cells.
      Title: Proteomic Analysis of Regulatory T Cells Reveals the Importance of Themis1 in the Control of Their Suppressive Function.
    10. Themis1 acts as a positive regulator of TCR signaling during thymocyte development by promoting Vav1 activity and Grb2 stability
      Title: Themis1 enhances T cell receptor signaling during thymocyte development by promoting Vav1 activity and Grb2 stability.
    Submit: New GeneRIF Correction See all GeneRIFs (23)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in T cell receptor signaling pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in T cell receptor signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within adaptive immune response IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within immune system process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within multicellular organism development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative T cell selection IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive T cell selection IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of COP9 signalosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell-cell junction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    protein THEMIS
    Names
    grb2-associating protein
    signaling phosphoprotein specific for T cells
    thymocyte selection pathway associated
    thymocyte-expressed molecule involved in selection
    thymus lymphocyte exclusively expressed

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001305663.1 → NP_001292592.1  protein THEMIS isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate 5' exon and lacks a 3' coding exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). The encoded isoform (2) has a shorter N-terminus and a shorter and distinct C-terminus, compared to isoform 1.
      Source sequence(s)
      AC153798, AC159472, AK040594, AK169558, BB638055
      UniProtKB/Swiss-Prot
      Q8BGW0
      Conserved Domains (1) summary
      pfam12736
      Location:1 → 177
      CABIT; Cell-cycle sustaining, positive selection,

    2. NM_001374699.1 → NP_001361628.1  protein THEMIS isoform 4

      Status: REVIEWED

      Source sequence(s)
      AC152983, AC159472
      Consensus CDS
      CCDS87977.1
      Related
      ENSMUSP00000101155.3, ENSMUST00000105516.9
      Conserved Domains (1) summary
      pfam12736
      Location:17 → 266
      CABIT; Cell-cycle sustaining, positive selection,

    3. NM_178666.6 → NP_848781.1  protein THEMIS isoform 1

      See identical proteins and their annotated locations for NP_848781.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AA967480, AK040594, AK169558, BB638055
      Consensus CDS
      CCDS23758.1
      UniProtKB/Swiss-Prot
      Q059Y0, Q3TEM0, Q3UU76, Q8BGP0, Q8BGW0, Q8C9S6
      Related
      ENSMUSP00000060129.5, ENSMUST00000056097.11
      Conserved Domains (1) summary
      pfam12736
      Location:17 → 266
      CABIT; Cell-cycle sustaining, positive selection,

    RNA

    1. NR_131208.1 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) has multiple differences, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AC152983, AC159472
      Related
      ENSMUST00000162202.8

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000076.7 Reference GRCm39 C57BL/6J

      Range
      28544356..28759816
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006512643.1 → XP_006512706.1  protein THEMIS isoform X1

      Conserved Domains (1) summary
      pfam12736
      Location:1 → 177
      CABIT; Cell-cycle sustaining, positive selection,
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8BGW0.1 GenPept UniProtKB/Swiss-Prot:Q8BGW0

    Gene LinkOut

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