Tjp2 tight junction protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tjp2provided by MGI
Official Full Name: tight junction protein 2provided by MGI
Primary source: MGI:MGI:1341872
See related: Ensembl:ENSMUSG00000024812 AllianceGenome:MGI:1341872
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ZO-2
Summary: Predicted to enable several functions, including cell adhesion molecule binding activity; protein C-terminus binding activity; and protein tyrosine kinase binding activity. Acts upstream of or within TNFSF11-mediated signaling pathway and negative regulation of osteoclast development. Located in nucleus; plasma membrane; and tight junction. Is expressed in several structures, including early embryo; genitourinary system; inner ear; neural ectoderm; and tooth. Human ortholog(s) of this gene implicated in autosomal dominant nonsyndromic deafness 51; disease of metabolism; and progressive familial intrahepatic cholestasis 4. Orthologous to human TJP2 (tight junction protein 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in lung adult (RPKM 24.4), small intestine adult (RPKM 19.3) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 B; 19 19.17 cM

Exon count:: 27

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (24071860..24202492, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (24094496..24225128, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Silenced LASP1 interacts with DNMT1 to promote TJP2 expression and attenuate articular cartilage injury in mice by suppressing TJP2 methylation.
Title: Silenced LASP1 interacts with DNMT1 to promote TJP2 expression and attenuate articular cartilage injury in mice by suppressing TJP2 methylation.
Protective Functions of ZO-2/Tjp2 Expressed in Hepatocytes and Cholangiocytes Against Liver Injury and Cholestasis.
Title: Protective Functions of ZO-2/Tjp2 Expressed in Hepatocytes and Cholangiocytes Against Liver Injury and Cholestasis.
The zonula occludens protein family regulates the hepatic barrier system in the murine liver.
Title: The zonula occludens protein family regulates the hepatic barrier system in the murine liver.
Based on results obtained in cultured epithelial cells transfected with ZO-2 mutated at a MET target lysine residue.
Title: The organophosphate pesticide methamidophos opens the blood-testis barrier and covalently binds to ZO-2 in mice.
Podocyte-specific deletion of the ZO-2 gene did not cause overt defects; however, double knockout of ZO-1 and ZO-2 genes accelerated the defects observed in ZO-1 knockout mice. These results suggest that ZO-2 plays supportive roles in the ZO-1-dependent regulation of podocyte filtration barrier.
Title: Effects of the differential expression of ZO-1 and ZO-2 on podocyte structure and function.
In the present study, we found that the protein level of ZO-2, a scaffolding tight junction protein, decreased in the bladder of OVX mice indicating a possible role in overactive bladder.
Title: A promising protein responsible for overactive bladder in ovariectomized mice.
Expression of Podocalyxin, which positively regulates the formation of microvilli and the apical membrane, is repressed in embryoid bodies lacking both ZO-1 and ZO-2 and this correlates with an aberrant submembranous localization of Ezrin.
Title: ZO-1 and ZO-2 are required for extra-embryonic endoderm integrity, primitive ectoderm survival and normal cavitation in embryoid bodies derived from mouse embryonic stem cells.
SNX27 associates with ZO-2 and modulates the epithelial tight junction.
Title: Sorting nexin 27 (SNX27) associates with zonula occludens-2 (ZO-2) and modulates the epithelial tight junction.
ZO-2 protects against podocyte dysfunction induced by adreiamycin. This action is regulated by ZO-2-mediated inhibition of the Wnt/beta-catenin signaling pathway
Title: Zona occludens-2 protects against podocyte dysfunction induced by ADR in mice.
the first PDZ domain of ZO-2 interacts with YAP2 to form complex; ZO-2 facilitates both nuclear translocation of YAP2 & pro-apoptotic function of YAP2; these activities of ZO-2 are PDZ-domain-dependent
Title: Functional complexes between YAP2 and ZO-2 are PDZ domain-dependent, and regulate YAP2 nuclear localization and signalling.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D19Mit107 (e-PCR), detects polymorphism
- UniSTS:128322

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cell adhesion molecule binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell-cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell-cell junction organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of endothelial intestinal barrier	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of endothelial intestinal barrier	ISO Inferred from Sequence Orthology more info
involved_in homotypic cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in intestinal absorption	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of osteoclast development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of tumor necrosis factor-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of blood-brain barrier permeability	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of blood-brain barrier permeability	ISO Inferred from Sequence Orthology more info
involved_in protein localization to cell-cell junction	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of membrane permeability	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
is_active_in bicellular tight junction	IBA Inferred from Biological aspect of Ancestor more info
located_in bicellular tight junction	ISO Inferred from Sequence Orthology more info
located_in cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cell-cell contact zone	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in gap junction	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in tight junction	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: tight junction protein ZO-2

Names: Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2); zona occludens protein 2; zonula occludens protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001198985.2 → NP_001185914.1 tight junction protein ZO-2 isoform 1

See identical proteins and their annotated locations for NP_001185914.1

Status: VALIDATED

Source sequence(s)

AC149224, AK028494, BM224980, BP767126, BQ176958, CB724174, CF736959, CN527767, CN529621

Consensus CDS

CCDS37939.2

UniProtKB/TrEMBL

A0A3B2WBH9, A0A3B2WCN9

Related

ENSMUSP00000156605.2, ENSMUST00000233027.2

Conserved Domains (5) summary

cd12027
Location:607 → 669

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:705 → 884

GuKc; Guanylate kinase homologues

smart00228
Location:311 → 388

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:32 → 117

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam08648
Location:219 → 283

DUF1777; Protein of unknown function (DUF1777)
NM_001360391.1 → NP_001347320.1 tight junction protein ZO-2 isoform 3

Status: VALIDATED

Source sequence(s)

AC107685, AC149224, BQ176958

UniProtKB/TrEMBL

A0A3B2WBH9

Conserved Domains (4) summary

cd12027
Location:611 → 673

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:709 → 888

GuKc; Guanylate kinase homologues

smart00228
Location:315 → 392

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:36 → 121

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_001360392.1 → NP_001347321.1 tight junction protein ZO-2 isoform 4

Status: VALIDATED

Source sequence(s)

AC107685, AC149224, BQ176958, BY073294

UniProtKB/TrEMBL

A0A3B2WBH9

Conserved Domains (4) summary

cd12027
Location:584 → 646

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:682 → 861

GuKc; Guanylate kinase homologues

smart00228
Location:288 → 365

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:9 → 94

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_001376368.1 → NP_001363297.1 tight junction protein ZO-2 isoform 2

Status: VALIDATED

Source sequence(s)

AC107685, AC149224

UniProtKB/Swiss-Prot

Q8K210, Q9Z0U1

UniProtKB/TrEMBL

A0A3B2WBH9

Related

ENSMUSP00000156896.2, ENSMUST00000232956.2

Conserved Domains (4) summary

cd12027
Location:584 → 646

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:682 → 861

GuKc; Guanylate kinase homologues

smart00228
Location:288 → 365

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:9 → 94

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_011597.5 → NP_035727.2 tight junction protein ZO-2 isoform 2

See identical proteins and their annotated locations for NP_035727.2

Status: VALIDATED

Source sequence(s)

AC149224, AK028494, BM224980, BP767126, BQ176958, BY032291, CB724174, CF736959, CN527767, CN529621

Consensus CDS

CCDS89365.1

UniProtKB/Swiss-Prot

Q8K210, Q9Z0U1

UniProtKB/TrEMBL

A0A3B2WBH9

Related

ENSMUSP00000097154.5, ENSMUST00000099558.5

Conserved Domains (4) summary

cd12027
Location:584 → 646

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:682 → 861

GuKc; Guanylate kinase homologues

smart00228
Location:288 → 365

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:9 → 94

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

24071860..24202492 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017318136.2 → XP_017173625.1 tight junction protein ZO-2 isoform X6

UniProtKB/TrEMBL

A0A3B2WBH9

Conserved Domains (4) summary

cd12027
Location:584 → 646

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:682 → 861

GuKc; Guanylate kinase homologues

smart00228
Location:288 → 365

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:9 → 94

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
XM_036161512.1 → XP_036017405.1 tight junction protein ZO-2 isoform X3

UniProtKB/Swiss-Prot

Q8K210, Q9Z0U1

UniProtKB/TrEMBL

A0A3B2WBH9

Related

ENSMUSP00000156728.3, ENSMUST00000233658.3

Conserved Domains (4) summary

cd12027
Location:584 → 646

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:682 → 861

GuKc; Guanylate kinase homologues

smart00228
Location:288 → 365

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:9 → 94

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
XM_030250861.2 → XP_030106721.1 tight junction protein ZO-2 isoform X5

UniProtKB/TrEMBL

A0A3B2WBH9

Conserved Domains (4) summary

cd12027
Location:607 → 669

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:705 → 884

GuKc; Guanylate kinase homologues

smart00228
Location:311 → 388

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:32 → 117

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
XM_006526904.5 → XP_006526967.1 tight junction protein ZO-2 isoform X1

UniProtKB/TrEMBL

A0A3B2WBH9

Conserved Domains (4) summary

cd12027
Location:625 → 687

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:723 → 902

GuKc; Guanylate kinase homologues

smart00228
Location:329 → 406

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:50 → 135

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
XM_006526909.3 → XP_006526972.1 tight junction protein ZO-2 isoform X4

UniProtKB/TrEMBL

A0A3B2WBH9

Conserved Domains (4) summary

cd12027
Location:611 → 673

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:709 → 888

GuKc; Guanylate kinase homologues

smart00228
Location:315 → 392

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:36 → 121

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
XM_006526907.3 → XP_006526970.1 tight junction protein ZO-2 isoform X2

UniProtKB/TrEMBL

A0A3B2WBH9

Conserved Domains (4) summary

cd12027
Location:625 → 687

SH3_ZO-2; Src homology 3 domain of the Tight junction protein, Zonula occludens protein 2

smart00072
Location:723 → 902

GuKc; Guanylate kinase homologues

smart00228
Location:329 → 406

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00992
Location:50 → 135

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...