GEO DataSets

Analysis of retinoblastoma (RB) ∆G/∆G mutant MEFs deprived of serum to induce quiescence. The mutant allele disrupts RB protein (pRB) interactions with the transactivation domain of E2F transcription factors. Results provide insight into pRB functions in cell cycle control independent of E2F.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 genotype/variation sets

Platform:

GPL6246

Series:

GSE54924

9 Samples

Download data: CEL

(Submitter supplied) The retinoblastoma protein (pRB) is best known for regulating cell proliferation through E2F transcription factors. In this report we investigate the properties of a targeted mutation that disrupts pRB interactions with the transactivation domain of E2Fs. Mice that carry this mutation endogenously (Rb1∆G) are defective in regulating E2F target genes. Surprisingly, cell cycle regulation in Rb1∆G/∆G MEFs strongly resembles that of wild type. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5641

Platform:

GPL6246

9 Samples

Download data: CEL

(Submitter supplied) Transcriptional profilling of 60 day old prostate tissue from Rb1F/F:Trp53F/F:PB-Cre4 or Rb1R654W/F:Trp53F/F:PB-cre4 mice versus wild type prostate tissue. Profiles used to compare deregulation of known E2F target genes in prostate tissue expressing a mutant Rb1 gene to tissue lacking Rb1 expression.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

4 Samples

Download data: TXT

(Submitter supplied) The LIM-only protein FHL2 acts as a transcriptional modulator that positively or negatively regulates multiple signaling pathways. We recently reported that FHL2 cooperates with CBP/p300 in the activation of ß-catenin/TCF target gene cyclin D1. In this paper, we demonstrate that FHL2 is associated with the cyclin D1 promoter at the TCF/CRE site, providing evidence that cyclin D1 is a direct target of FHL2. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3344

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of spontaneously immortalized embryonic fibroblasts (EFs) lacking the LIM-only protein FHL2. FHL2 deficiency results in reduced proliferative capacity of spontaneously immortalized EFs. Results provide insight into the role of FLH2 in cell cycle and cell proliferation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE10902

6 Samples

Download data: CEL

(Submitter supplied) Retinoblastoma gene (Rb1) is required for proper cell cycle exit in the developing mouse inner ear and its deletion in the embryo leads to proliferation of sensory progenitor cells that differentiate into hair cells and supporting cells. In the Pou4f3-Cre:Rb1 flox/flox (Rb1 cKO) inner ear, utricular hair cells differentiate and survive into adulthood whereas differentiation and survival of cochlear hair cells are impaired. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL339 GPL340

24 Samples

Download data: CEL

(Submitter supplied) The mammalian heart is incapable of regenerating a sufficient number of cardiomyocytes to ameliorate the loss of contractile muscle after acute myocardial injury, often resulting in heart failure. Several reports have demonstrated that mononucleated (MoNuc) cardiomyocytes are more responsive to pro-proliferative stimuli than are binucleated (BiNuc) cardiomyocytes. However, techniques to isolate and characterize these two different cardiomyocyte populations have been lacking. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

18 Samples

Download data: CSV

(Submitter supplied) E2F transcription factors control the expression of a large network of cell cycle genes and are essential for S-phase entry. Cancers often demonstrate upregulation of E2F target gene expression, which can be partially explained by loss of the G1/S checkpoint and increased percentages of replicating cells. However, we now demonstrate that cycling individual neoplastic cells can display abnormally high levels of E2F-dependent transcription using single cell RNA sequencing. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

4 Samples

Download data: CSV

(Submitter supplied) The goal of the experiment was to identify and compare at the genome-wide level the targets of E2FA, E2FB and E2FC, the three canonical E2Fs, and those of their binding partner RBR.

Organism:

Arabidopsis thaliana

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19580

7 Samples

Download data: BIGWIG, BROADPEAK, NARROWPEAK