GEO DataSets

Analysis of WAT from high fat diet-fed Smad3-/- mice and anti-TGF-beta antibody-treated, diet-induced obese (DIO) mice. Smad3 loss protects against DIO and diabetes. TGF-b signaling blockade protects against obesity and diabetes. Results provide insight into molecular mechanisms underlying diabetes.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 genotype/variation, 4 protocol sets

Platform:

GPL1261

Series:

GSE28598

12 Samples

Download data: CEL, CHP

(Submitter supplied) Imbalances in glucose and energy homeostasis are at the core of the worldwide epidemic of obesity and diabetes. Here, we illustrate an important role of the TGF-beta/Smad3 signaling pathway in regulating glucose and energy homeostasis. Smad3 deficient mice are protected from diet-induced obesity and diabetes. Interestingly, the metabolic protection is accompanied by Smad3-/- white adipose tissue acquiring the bioenergetic and gene expression profile of brown fat/skeletal muscle. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3985

Platform:

GPL1261

12 Samples

Download data: CEL, CHP

(Submitter supplied) The β-adrenergic receptor signaling pathway is a major component of adaptive thermogenesis in brown and white adipose tissue during cold acclimation. The β-AR activation highly induces transcriptional coactivator PGC-1α and its splice variant N-terminal (NT)-PGC-1α, promoting the transcription program of mitochondrial biogenesis and thermogenesis. In the present study, we evaluated the role of NT-PGC-1α in brown adipocyte energy metabolism by genome-wide profiling of NT-PGC-1α-responsive genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

4 Samples

Download data: TXT

(Submitter supplied) Mitochondria play an essential role in the ability of brown fat to generate heat, and the PGC-1 coactivators control several aspects of mitochondrial biogenesis. To investigate their specific roles in brown fat cells, we generated immortal preadipocyte lines from the brown adipose tissue of mice lacking PGC-1α. We could then efficiently knockdown PGC-1β expression by shRNA expression. Loss of PGC-1α did not alter brown fat differentiation but severely reduced the induction of thermogenic genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2123

Platform:

GPL1261

6 Samples

Download data

(Submitter supplied) To investigate the specific role of PGC-1 coactivators in brown fat cells, we generated immortal preadipocyte lines from the brown adipose tissue of mice lacking PGC-1alpha. We could then efficiently knockdown PGC-1beta expression by shRNA expression. Loss of PGC-1alpha did not alter brown fat differentiation but severly reduced the induction of thermogenic genes. In order to assess the specific requirement for PGC-1α in the global transcriptional response to cAMP, we used Affymetrix arrays to compare the sets of genes induced in response to a 4 hr dbcAMP treatment in differentiated wt and KO cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2149

Platform:

GPL1261

8 Samples

Download data

Analysis of PGC-1alpha null brown adipocytes treated with cAMP for 4 hours. PGC-1alpha is required for the thermogenic function of brown fat cells, and cAMP plays a role in thermogenesis. Results provide insight into the role of PGC-1alpha in the global transcriptional response to cAMP.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE5041

8 Samples

Download data

Analysis of brown fat cells lacking PGC-1alpha or both PGC-1alpha and PGC-1beta. PGC-1alpha is required for the thermogenic function of brown fat cells, and PGC-1beta is the closest homolog of PGC-1alpha. Results provide insight into the specific roles of PGC-1alpha and PGC-1beta in brown fat cells.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE5042

6 Samples

Download data

(Submitter supplied) The goal of this study was to identify genes in C2C12 myoblasts whose expression was altered by overexpression of Smad3.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

4 Samples

Download data: CEL

(Submitter supplied) The goal of this experiment was to examine the sex-dependent effect of Siah2 in brown adipose tissue

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6885 GPL6887

18 Samples

Download data: TXT

(Submitter supplied) The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Keywords: tissue comparison from mice

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2813

Platform:

GPL1261

6 Samples

Download data: CEL, CHP, DCP, TXT

Comparison of brown and white adipose tissues. Brown fat cells are specialized to dissipate energy and can counteract obesity. Results provide insight into the molecular mechanisms controlling brown fat cell determination.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 tissue sets

Platform:

GPL1261

Series:

GSE8044

6 Samples

Download data: CEL, CHP, DCP, TXT

(Submitter supplied) Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype we performed DNA microarray analysis on white adipose tissue (WAT) from PeriA transgenic (Tg) and control wildtype (WT) mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL

(Submitter supplied) The processes by which mitochondria respond to cellular energy demands brought on by physiologic and pathophysiologic stimuli are essential to cellular adaptation and organismal survival. Disrupted mitochondrial function is implicated in several human disease states, underscoring the need to elucidate the molecular mechanisms that control mitochondrial function and metabolism. We previously described a patient with a gain-of-function mutation in Site-1 Protease (S1P) who exhibited idiopathic hyperCKemia, myoedema, and altered muscle mitochondrial morphology. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

18 Samples

Download data: TXT